5 Based on your experimental results, do nongerminating peas undergo cell respiration? 6 List three factors that could possibly affect cell respiration rates in germinating and/or nongerminating peas.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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EXERCISE
10.1
Investigating Cellular Respiration
Using Vernier Sensors
Cell respiration refers to the process of converting the chemical
energy of organic molecules into a form immediately usable by
organisms. Glucose may be oxidized completely if sufficient
oxygen is available by the following equation:
C6H12O6 + 6 O₂(g)
->
6 H₂O + 6 CO₂(g) + energy
All organisms, including plants and animals, oxidize glucose
for energy. Often, this energy is used to convert ADP and
phosphate into ATP. It is known that peas undergo cell
respiration during germination.
In Procedure 1, you will use a CO₂ gas sensor to determine
the respiration rate of peas.
ABBON
CARBON
DIOXIDE
Procedure 1
Respiration Rate of Peas
1 If your CO₂ gas sensor has a switch, set it to the low
(0-10,000 ppm) setting. Connect the CO₂ gas sensor to
the data-collection interface.
2 Start the data-collection program. Allow 90 seconds for
the sensor to warm up.
3 Prepare the peas for data collection.
a Obtain 25 germinating peas and blot them dry with a
paper towel.
b Determine and record the mass of the peas. (15.508)
c Place the peas in the respiration chamber.
d Place the shaft of the CO₂ gas sensor in the opening
of the respiration chamber and lay flat, as shown in
Figure 10.2.
Very Important: Do not place the sensor directly into any
liquid. The sensor is intended only for measuring gaseous,
not aqueous, CO₂ concentration.
4 Pause 60 seconds, and then start data collection. Data
will be collected for 600 seconds.
5 When data collection has finished, remove the CO₂
gas sensor from the respiration chamber. Return the
peas to the designated location. Rinse the respiration
chamber with water and then empty it. Repeat this
rinsing procedure two more times. Thoroughly dry the
inside of the chamber with a paper towel.
6 Use the linear regression function to determine the
respiration rate, which is the slope of the line, m.
Record the respiration rate in ppm CO₂/s.
CH:CO2
CH₂ - Temp
485ppm
22-2°C
60
Transcribed Image Text:EXERCISE 10.1 Investigating Cellular Respiration Using Vernier Sensors Cell respiration refers to the process of converting the chemical energy of organic molecules into a form immediately usable by organisms. Glucose may be oxidized completely if sufficient oxygen is available by the following equation: C6H12O6 + 6 O₂(g) -> 6 H₂O + 6 CO₂(g) + energy All organisms, including plants and animals, oxidize glucose for energy. Often, this energy is used to convert ADP and phosphate into ATP. It is known that peas undergo cell respiration during germination. In Procedure 1, you will use a CO₂ gas sensor to determine the respiration rate of peas. ABBON CARBON DIOXIDE Procedure 1 Respiration Rate of Peas 1 If your CO₂ gas sensor has a switch, set it to the low (0-10,000 ppm) setting. Connect the CO₂ gas sensor to the data-collection interface. 2 Start the data-collection program. Allow 90 seconds for the sensor to warm up. 3 Prepare the peas for data collection. a Obtain 25 germinating peas and blot them dry with a paper towel. b Determine and record the mass of the peas. (15.508) c Place the peas in the respiration chamber. d Place the shaft of the CO₂ gas sensor in the opening of the respiration chamber and lay flat, as shown in Figure 10.2. Very Important: Do not place the sensor directly into any liquid. The sensor is intended only for measuring gaseous, not aqueous, CO₂ concentration. 4 Pause 60 seconds, and then start data collection. Data will be collected for 600 seconds. 5 When data collection has finished, remove the CO₂ gas sensor from the respiration chamber. Return the peas to the designated location. Rinse the respiration chamber with water and then empty it. Repeat this rinsing procedure two more times. Thoroughly dry the inside of the chamber with a paper towel. 6 Use the linear regression function to determine the respiration rate, which is the slope of the line, m. Record the respiration rate in ppm CO₂/s. CH:CO2 CH₂ - Temp 485ppm 22-2°C 60
5 Based on your experimental results, do nongerminating peas undergo cell respiration?
6 List three factors that could possibly affect cell respiration rates in germinating and/or nongerminating peas.
Transcribed Image Text:5 Based on your experimental results, do nongerminating peas undergo cell respiration? 6 List three factors that could possibly affect cell respiration rates in germinating and/or nongerminating peas.
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