5'-AAGCGGGTGTGAGGAGCGCGGCAGCTCAGGTACCCCCGGCCAGGCGCG-3' 31-TTCGCCCACACTCCTCGCGCCGT CGAGTCCATGGGGGCCGGTCCGCGC-5' What primer sequences do you need to amplify this piece of DNA? Select one: 5'-ACACCCGCTT-3' and 5'-GCCAGGCGCG-3' 5'-TTCGCCCACA-3' and 5'-GCGCGGACCG-3' 5'-TGTGGGCGAA-3 and 5'-CGGTCCGCGC-3' 5'-AAGCGGGTGT-3 and 5'-CGCGCCTGGC-3' 5'-ATGGGGGCCG-3 and 5'-AGCTCAGGTA-3'
5'-AAGCGGGTGTGAGGAGCGCGGCAGCTCAGGTACCCCCGGCCAGGCGCG-3' 31-TTCGCCCACACTCCTCGCGCCGT CGAGTCCATGGGGGCCGGTCCGCGC-5' What primer sequences do you need to amplify this piece of DNA? Select one: 5'-ACACCCGCTT-3' and 5'-GCCAGGCGCG-3' 5'-TTCGCCCACA-3' and 5'-GCGCGGACCG-3' 5'-TGTGGGCGAA-3 and 5'-CGGTCCGCGC-3' 5'-AAGCGGGTGT-3 and 5'-CGCGCCTGGC-3' 5'-ATGGGGGCCG-3 and 5'-AGCTCAGGTA-3'
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Please answer asap and type your answer and do not copy from anywhere
Transcribed Image Text:You are working in a molecular biology lab and have recently received a research grant to
develop a PCR-based DNA test for a specific chromosome mutation found in cancer cells. In
these cells, a translocation in the middle of two genes causes the production of an abnormal
fusion protein that promotes uncontrolled cell proliferation and thus the development of
cancer. Your objective is to amplify the following DNA sequence surrounding the fusion site
using PCR:
5'-AAGCGGGTGTGAGGAGCGCGGCAGCTCAGGTACCCCCGGCCAGGCGCG-3'
31-TTCGCCCACACTCCT CGCGCCGTCGAGTCCATGGGGGCCGGTCCGCGC-5'
What primer sequences do you need to amplify this piece of DNA?
Select one:
O 5'-ACACCCGCTT-3 and 5'-GCCAGGCGCG-3¹
O 5'-TTCGCCCACA-3' and 5'-GCGCGGACCG-3'
5'-TGTGGGCGAA-3' and 5'-CGGTCCGCGC-3'
5'-AAGCGGGTGT-3' and 5'-CGCGCCTGGC-3'
O 5'-ATGGGGGCCG-3' and 5'-AGCTCAGGTA-3'
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