5-11 Decarboxylation test 1. Write the molecular structure of the carboxyl group. 2. What promotes the production of the decarboxylase enzyme in decarboxylase- positive microorganisms? 3. What process causes the decrease of pH in the medium in the decarboxylation test?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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bio 2 from Lab Manual 4 TH edition Leboffe and Pierce 

**5-11 Decarboxylation Test**

1. **Write the molecular structure of the carboxyl group.**

2. **What promotes the production of the decarboxylase enzyme in decarboxylase-positive microorganisms?**

3. **What process causes the decrease of pH in the medium in the decarboxylation test?**

4. **What is the purpose of the addition of the mineral oil layer after inoculation in the decarboxylation test?**

5. **Why does the decarboxylase-positive tube turn yellow before it turns purple?**

6. **This decarboxylation test cannot be performed successfully on organisms that cannot ferment glucose. Explain why.**

7. **What amino acid is being decarboxylated in this test?**

**RESULTS / INTERPRETATION**

Draw out your results. Be sure to properly label the test tubes with the organism, color results, and describe if the results were positive or negative and interpret the results.

_**Control:**_

Below this section, there are three rectangles side by side representing test tubes where you can draw and label your results.
Transcribed Image Text:**5-11 Decarboxylation Test** 1. **Write the molecular structure of the carboxyl group.** 2. **What promotes the production of the decarboxylase enzyme in decarboxylase-positive microorganisms?** 3. **What process causes the decrease of pH in the medium in the decarboxylation test?** 4. **What is the purpose of the addition of the mineral oil layer after inoculation in the decarboxylation test?** 5. **Why does the decarboxylase-positive tube turn yellow before it turns purple?** 6. **This decarboxylation test cannot be performed successfully on organisms that cannot ferment glucose. Explain why.** 7. **What amino acid is being decarboxylated in this test?** **RESULTS / INTERPRETATION** Draw out your results. Be sure to properly label the test tubes with the organism, color results, and describe if the results were positive or negative and interpret the results. _**Control:**_ Below this section, there are three rectangles side by side representing test tubes where you can draw and label your results.
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