4. The study of conditions that optimize the association of proteins in solution guides the design of protocols for formation of large crystals. It is important to characterize protein dimerization (2P - because the process is considered to be the rate-determining step in the growth of crystals of many proteins. Consider the variation with ionic strength (I) of the rate constant (k) of dimerization in aqueous solution of an ionic protein P at 298 K: P2), I/Io 0.0100 0.0150 0.0200 0.0250 0.0300 0.0350 k/ko 8.10 13.30 20.50 27.80 38.10 52.00 Note: Io = 1 mol/L, ko = 1 L/(mol•s). (a) What effect is this, and what is its physical origin? (b) Determine the charge state of this protein, P. Note: One needs to use all data points.

4. The study of conditions that optimize the association of proteins in solution guides the design of protocols for formation of large crystals. It is important to characterize protein dimerization (2P - because the process is considered to be the rate-determining step in the growth of crystals of many proteins. Consider the variation with ionic strength (I) of the rate constant (k) of dimerization in aqueous solution of an ionic protein P at 298 K: P2), I/Io 0.0100 0.0150 0.0200 0.0250 0.0300 0.0350 k/ko 8.10 13.30 20.50 27.80 38.10 52.00 Note: Io = 1 mol/L, ko = 1 L/(mol•s). (a) What effect is this, and what is its physical origin? (b) Determine the charge state of this protein, P. Note: One needs to use all data points.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Related questions

Q: (3) The partition coefficient Kp for the following is 2.52 Given 500 mL of an aqueous solution cont…

A: The solubility of a substance in a solvent to yield a solution depends on the rule 'like dissolves…

Q: R. Atkinson (J. Phys. Chem. Ref. Data 26, 215 (1997)) has reviewed a large set of rate constants…

Q: 1. Bulk Ti-Al alloy was subjected to 1100 K oxidation for 5 hr, which led to the growth of 3.5…

A: XRD (X-ray Diffraction):X-ray Diffraction is a technique used to analyze the crystal structure of…

Q: weight of 1000.0 g mol- was used. What concentration of PEG is needed to create an aqueous solution…

A: In Osmosis, the osmotic pressure of a solution is the excess pressure that must be imparted to the…

Q: Equilibrium constants for reactions can be determined from partition functions. For a generic…

A: The equilibrium constant for the following reaction at 298 K is needed to be determined. H2O + DCl…

Q: Account for the hydrophobic effect and discuss its manifestations.

A: Hydrophobic is composed of two words hydro and phobic where hydro means water and phobic comes from…

Q: 6. [15] The diffusion coefficient of sucrose molecule in water is 5.22×10-¹0 m² s¹ at 25°C. How long…

Q: A political candidate fighting for a smoke-free environment conducts an experiment in a room…

A: Concentration of CO introduced in the room = 4% =0.04 Volume of Room = 1400 ft3 Rate of CO inside…

Q: The equilibrium constant for the reaction N2(g) + O2(g) 2 NO(g) is 1.7 x 10-1 at an elevated…

A: Let us consider a following reaction aA + bB = cC + dD then reaction Q is defined as Q = (pC)c x…

Q: 2. The investigation of the reaction represented by the equation: X (g) + Y (g) → Z (g), allowed to…

A: Given:Temperature = Rate constant = The Arrhenius equation describes the temperature dependence of…

Q: 5) Starting from the expression G, – Cy = T (O"/aT), (®V/at), ' use the appropriate relations…

A: The given expression is the thermodynamic relation between specific heat capacity at constant…

Q: Solute S has a partition co-efficient of 3.0 between water (Phase 1) and Chloroform (Phase 2). (a)…

Q: The rate of an enzyme-catalyzed reaction is 2.26×10° times faster than the rate of the uncatalyzed…

Q: Write the Gibbs-Helmholtz equation and show its relation with reaction spontaneity.

Q: In the context of molecular modelling, several classes of force fields have been developed for the…

A: Detailed explanation shown in explanation section.Explanation:In molecular modelling, The energy of…

Q: Acetic acid is a weak acid that partially dissociated in water according to equation B1-2, with an…

Q: A mixture of isobutylene (CH3)CCH2,0.400 bar partial pressure and HCl , 0.600 bar partial pressure…

Q: Calculate the standard reaction entropy at 298k of: (a) Zn(s) +Cu (aq)Zn (aq) + Cu(s) (b) CH,O(s)…

A: Entropy is the measure of randomness. And molar entropy is the entropy of 1 mole of the substance…

Q: - During production, transport and use of natural gas, some is inevitably leaked into the atmosphere…

A: Second order reactions can be defined as chemical reactions wherein the sum of the exponents in the…

Q: In an extraction process, a solute species is partitionedbetween two immiscible solvents. Suppose…

A: Introduction- Solvent extraction is a process where 2 immiscible solvents are separated along with…

Q: Construct the expression for Ksp for solid Ba3(PO4)2 in aqueous solution. Вaз(РOд)2(s) 3 З Ва" (aq)…

A: The solubility equilibria for Ba3PO42 is as follows: Ba3PO42(s) ⇌ 3Ba2+(aq) + 2PO43-(aq) Let S is…

Q: P5B2 In a study of the properties of an aqueous solution of Th(NO3) by A. Apelblat, D. Azoulay, and…

Q: The half-life is 123 min at 15.0°C. If 138.0 mL of a 0.105 M NH₂NO₂ solution is allowed to…

A: Given, t1/2 = 123 min. The volume of NH2NO2 = 138.0 mL The concentration of NH2NO2 = 0.105 M The…

Q: Determine enthalpy of activation, entropy of activation, free energy of activation (25 degC)

A: Given:

Q: Protein Denatured a. Show that the fraction of denatured macromolecules, 0, is related to the…

A: Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

Question

4. The study of conditions that optimize the association of proteins in solution guides the design of
protocols for formation of large crystals. It is important to characterize protein dimerization (2P –→ P2),
because the process is considered to be the rate-determining step in the growth of crystals of many
proteins. Consider the variation with ionic strength (I) of the rate constant (k) of dimerization in aqueous
solution of an ionic protein P at 298 K:
I/Io
0.0100
0.0150
0.0200
0.0250
0.0300
0.0350
k/ko
8.10
13.30
20.50
27.80
38.10
52.00
Note: Io = 1 mol/L, ko = 1 L/(mol•s).
(a) What effect is this, and what is its physical origin?
(b) Determine the charge state of this protein, P. Note: One needs to use all data points.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

Step 2

Step 3

Step 4

Step 5

Step by step

Solved in 5 steps with 2 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

6. Use data from Table 2C.3 and 2C.4, to calculate the standard reaction at 298k of (i) (ii) (iii) 2CH;CHO (g) + O2 (g) -> 2 CH3COOH (I) 2 AgCI (s) + Br2 (1) -> 2 AgBr(s) + Cl2 (g) Hg (I) + Cl2 (g) -> HgCl2 (s)
Iodine is sparingly soluble in water but much more so in carbon tetrachloride. The equilibrium constant, also called the partition coefficient, for the distribution of I2 between these two phases I2(aq) =12(CCI4) is 83 at 20°C. (a) A student adds 0.028 L of CCI4 to 0.213 L of an aqueous solution containing 0.037 g I2. The mixture is shaken and the two phases are then allowed to separate. Calculate the fraction of I2 remaining in the aqueous phase. 4.0 (b) The student now repeats the extraction of I2 with another 0.028 L of CCI4. Calculate the fraction of I2 from the original solution that remains in the aqueous phase. 4.0 (c) Compare the result in (b) with a single extraction using 0.056 L of CCI4. Comment on the difference. After a single extraction using 0.056 L of CCI4, the fraction of I2 that remains in the aqueous phase is 40 This result is larger than the result in (b), which means that a single large extraction is not as efficient as two separate smaller extractions.
1. After 3 hours, Ibuprofen is at 100mg 2. m(t)=400e^−0.462t, K=0.462 Please answer question 5.
Pls help ASAP.
Draw a Langmuir isotherm and properly label the axes! Write down the equation defining the isotherm and identify the symbols in it! What conditions must be satisfied for the equation top be valid?
6.
A7
3) An empirical function that might be used to represent the molecular energy is E=0 一 B where A and B are positive constants. Find A and B in terms of the equilibrium separation Req and the dissociation energy Eo.