4. The enzyme, Xase (hypothetical, has the following kinetics: k1 k2 S+E ES + E+P k-1 k1 = 10 ° M1 s1 k-1 = 4.4 x 104 s1 k2 = 103 s a. what is the value of the Michaelis constant for Xase ? b. At an enzyme concentration of 10-6 M, what will be the initial rate of product formation at a substrate concentration of 10-3 M ?

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4. The enzyme, Xase (hypothetical, has the following kinetics:
k1
k2
S+E
ES
+ E+P
k-1
k1 = 10° M1 s1
k-1 = 4.4 x 104 s1
k2 = 103 s1
a. what is the value of the Michaelis constant for Xase ?
b. At an enzyme concentration of 10-6 M, what will be the initial rate of product
formation at a substrate concentration of 10-3 M ?
Transcribed Image Text:4. The enzyme, Xase (hypothetical, has the following kinetics: k1 k2 S+E ES + E+P k-1 k1 = 10° M1 s1 k-1 = 4.4 x 104 s1 k2 = 103 s1 a. what is the value of the Michaelis constant for Xase ? b. At an enzyme concentration of 10-6 M, what will be the initial rate of product formation at a substrate concentration of 10-3 M ?
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