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- 8. The brick red color formed in a positive result of Benedict's test comes from A. the oxidation of copper ions B. the reduction of copper ion C. the reduction of carbonyl group D. the oxidation of carboxyl group E. the breakdown of glycosidic linkage 9. The following molecule is a но H Н-С—N — С H H H H-N-C -C-0-P-0-C -H H H H H A. triacylglycerol B. steroid hormone C. phospholipid D. o-6 fatty acid E. cholesterol 10. Which of the following statements about nucleic acid is INCORRECT? A. Coenzyme A is a protein, not nucleic acid B. NAD is an example of nucleic acid C. Adenine base is the same in ribonucleotide and deoxyribonucleotide D. Helix is an example of the secondary structure of nucleic acid E. Hydrogen bond can be found in DNA 11. The nucleoside consists of a D-deoxyribose and a cytosine base is called A. cytidine B. deoxycytidine C. deoxycytidylate D. deoxyribosylcytosine E. deoxyribosylcytidine2. During SDS PAGE, Tighnari loaded the purified protein extract from redcrest and padisarah, another sample that he is working with for his dissertation. Note that the isolated proteins from padisarah have molecular weights similar to that of the proteins isolated from redcrest. The results of the SDS-PAGE are shown in Figure D.2. SDS-PAGE w/o ß-mercaptoethanol LANE 1 B D LANE 2 PHILGAR SDS-PAGE w/ p-mercaptoethanol LANE 1 !!! LANE 2 Figure D.2. Electrophoretogram obtained after SDS-PAGE of the purified protein extract from redcrest and padisarah.1. Which of the following statement/s is/are TRUE for the protein sample?* The sample will give a positive result to Biuret test. All of A, B and C The sample will give a positive result to Ninhydrin test. The sample will give a positive result to Xanthoproteic test. 2. Extremely high pH causes folding of the protein molecules. * The statement is CORRECT. The statement is INCORRECT. 3.Negative with Biuret Test but positive with Ninhydrin Test, Xanthoproteic Test and Millon’s Test Glycine Tryptophan Tyrosine Methionine Albumin
- 1. Which of the following organisms would you use to change alkaline conditions to acidic conditions? a. B. cereus-VP + b. S. aureus- Glucose metabolism test + c. E. coli- Decarboxylase + d. P. vulgaris- Simmon's citrate + e. E. aerogenes- Gelatinase + 2. Which of the following results is similar to your carbohydrate metabolism tests results when bacterial metabolic byproducts initiated a color change of red to yellow in the agar? a. B. cereus-MR + b. S. aureus-VP+ c. E. coli- Decarboxylase + d. P. vulgaris- Simmon's citrate + e. E. aerogenes- Gelatinase + 3. After an acid-fast stain, a gram positive organism would be a. blue b. green c. cranberry red d. yellow e. orange 4. Your patient is on a diet where he consumes a lot of protein. Due to this diet and the over consumption of proteins, he is dangerously close to his blood becoming acidic. So therefore, which of the following did you administer (hypothetically) to buffer the acids? a. B. cereus: VP b. S. aureus: Decarboxylase + c. L.…What disadvantages can be seen in broiler chickens with soybean meal and 30% barley in their feeds that have not been sufficiently heating treated?2. Consider the following paragraph from a peer-reviewed publication detailing the extraction and isolation of a lectin: "30 g of dry S. sclarea seeds were ground in a Waring blender and the meal was stirred overnight in 400 ml of Phosphate Buffered Saline (PBS), at 4 °C. The solution was centrifuged at 20,000 X g for 30 min, and the pellet was re-extracted by mixing with another 400 ml of PBS, overnight at 4°C. The two supernatants were combined and frozen at -20 °C. After thawing, the insoluble material was spun down (3,500 X g for 30 min), and the clear supernatant (crude extract) was precipitated with 50% (v/v) cold ethanol at 4 °C. After centrifugation (20,000 X g for 30 min), the pellet was discarded, and the supernatant was further precipitated with cold ethanol up to 80% (v/v) at 4 °C. After one night in the cold room, the solution was centrifuged (20,000 X g for 30 min), the supernatant was discarded, and the pellet was re-dissolved in water, dialyzed for 3 days at 4 °C…
- Part a. You making 30 mo of agarose gel. Sybersafe A concentration 1000x. How much you will add......ul to the gel. b. You will be loading 20 your diagnostic digest onto your gal. The loading dye you have been provided is 5x concentrated. You will add..... ? ul to your sample before loading your gel.i don’t understand can you help meExample of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.
