3. Why did a mixture of 2.00 mL of pH 7.00 phosphate buffer, 0.900 mL of deionized water and 0.100 mL of 0.0300M p-nitrophenylacetate in acetonitrile have an A400 of approximately 0.03 at zero time? (Day 1, Run 1)

3. Why did a mixture of 2.00 mL of pH 7.00 phosphate buffer, 0.900 mL of deionized water and 0.100 mL of 0.0300M p-nitrophenylacetate in acetonitrile have an A400 of approximately 0.03 at zero time? (Day 1, Run 1)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

2. Why is there a difference between the stock solution concentration of chymotrypsin
calculated from zero time y-intercepts versus the stock solution label information?
3. Why did a mixture of 2.00 mL of pH 7.00 phosphate buffer, 0.900 mL of deionized water
and 0.100 mL of 0.0300M p-nitrophenylacetate in acetonitrile have an A400 of
approximately 0.03 at zero time? (Day 1, Run 1)

7. Discuss the effect of the pH change on the steady state deacylation rate; were your
observations consistent with the proposed mechanism? Explain your answer.

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