Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
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which one matches?
Worksheet: Crime Scene Electrophoresis
Type your responses to the questions below, including a labeled picture of your gel.qu'
1. When you started your gel, you had to make sure that you oriented it so it would "run to red."
What does this mean, and why does DNA tend to travel toward the "red" pole?
2. Suppose you loaded lane 1 of a gel with a 1000 bp PCR product, and lane 2 with a 1500 bp
PCR product. Which lane would have a band that travels farther on the gel, and why?
3. If any of your samples did not generate PCR products, explain why you think that might be.
4. Include a picture of your gel, with the allele ladder markers and each well clearly labelled.
What is the genotype of each of your samples?
5. Does the Crime Scene DNA sample have a genotype that matches any of the suspects? If so,
Badg wong tail-dead (g) ID
sestig (dendaaye) 2
penda
6. What does this result tell you about which suspects are included in the investigation?
Excluded? Explain your answer.
7. Imagine that each allele at the BXP007 locus is found at exactly the same frequency in a
population. Since there are 8 possible alleles at the BXP007 locus, what is the frequency of any
one allele from this locus in this population?
noizivibanda (oitoti) 14
to the cl](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0c1d0d01-ade3-4f63-bcf8-3821e33828ca%2F4990d698-de55-4b7d-931d-8d27c0e68f29%2Fdciyswl_processed.jpeg&w=3840&q=75)
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