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3) Use the Michaelis-Menten equation to answer the following questions about the relationship between reaction velocity and substrate concentration. a) At what substrate concentration would an enzyme with a Keat of 30.0 s¹ and a KM of 0.0050 M operate at one-quarter of its maximum rate? b) Determine the fraction of Vmax that would be obtained at the following substrate concentrations: ½ KM, 2KM, 10KM c) An enzyme that catalyzes the reaction X + Y is isolated from two bacterial species. The enzymes have the same Vmax but different KM values. Enzyme A has a KM of 2 μM, and Enzyme B has a KM of 0.5 μM. The plot shows the kinetics of reactions carried out with the same concentration E of each enzyme and with [X] 1 μM. Which curve corresponds to which enzyme? Time

3) Use the Michaelis-Menten equation to answer the following questions about the relationship between reaction velocity and substrate concentration. a) At what substrate concentration would an enzyme with a Keat of 30.0 s¹ and a KM of 0.0050 M operate at one-quarter of its maximum rate? b) Determine the fraction of Vmax that would be obtained at the following substrate concentrations: ½ KM, 2KM, 10KM c) An enzyme that catalyzes the reaction X + Y is isolated from two bacterial species. The enzymes have the same Vmax but different KM values. Enzyme A has a KM of 2 μM, and Enzyme B has a KM of 0.5 μM. The plot shows the kinetics of reactions carried out with the same concentration E of each enzyme and with [X] 1 μM. Which curve corresponds to which enzyme? Time

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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3) Use the Michaelis-Menten equation to answer the following questions about the relationship between reaction velocity and substrate concentration. a) At what substrate concentration would an enzyme with a Keat of 30.0 s¹ and a KM of 0.0050 M operate at one-quarter of its maximum rate? b) Determine the fraction of Vmax that would be obtained at the following substrate concentrations: ½ KM, 2KM, 10KM c) An enzyme that catalyzes the reaction X + Y is isolated from two bacterial species. The enzymes have the same Vmax but different KM values. Enzyme A has a KM of 2 μM, and Enzyme B has a KM of 0.5 μM. The plot shows the kinetics of reactions carried out with the same concentration E of each enzyme and with [X] 1 μM. Which curve corresponds to which enzyme? Time
Transcribed Image Text:3) Use the Michaelis-Menten equation to answer the following questions about the relationship between reaction velocity and substrate concentration. a) At what substrate concentration would an enzyme with a Keat of 30.0 s¹ and a KM of 0.0050 M operate at one-quarter of its maximum rate? b) Determine the fraction of Vmax that would be obtained at the following substrate concentrations: ½ KM, 2KM, 10KM c) An enzyme that catalyzes the reaction X + Y is isolated from two bacterial species. The enzymes have the same Vmax but different KM values. Enzyme A has a KM of 2 μM, and Enzyme B has a KM of 0.5 μM. The plot shows the kinetics of reactions carried out with the same concentration E of each enzyme and with [X] 1 μM. Which curve corresponds to which enzyme? Time
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