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2,4-dinitrophenyl hydrazine reacts with the carbonyl groups of ketone molecules to produce a coloured compound as per practical 3. H H C=0 + H₂N-N- H i) ii) O₂N -NO2 H₂O O₂N R -NO2 Provide the balanced equation of the reaction as it occurs in the pentose phosphate pathway (do not show any structures). Analyze the 6-phosphogluconate dehydrogenase reaction. Name the chemical reaction that the substrate of the reaction is subjected to in the pentose phosphate pathway? iii) Name the intermediate and the functional group of the reaction outlined in i) that reacts with 2,4 dinitrophenyl hydrazine. iv) indicate whether the reaction as outlined in i) would generate a 'high' or 'low' 510 nm absorbance reading in the presence of NADP+ or in the presence of NADPH, respectively. Justify your answer in a single sentence. Absorbance 510 nm in presences of NADP+: Absorbance 510 nm in presences of NADPH: Justification:

2,4-dinitrophenyl hydrazine reacts with the carbonyl groups of ketone molecules to produce a coloured compound as per practical 3. H H C=0 + H₂N-N- H i) ii) O₂N -NO2 H₂O O₂N R -NO2 Provide the balanced equation of the reaction as it occurs in the pentose phosphate pathway (do not show any structures). Analyze the 6-phosphogluconate dehydrogenase reaction. Name the chemical reaction that the substrate of the reaction is subjected to in the pentose phosphate pathway? iii) Name the intermediate and the functional group of the reaction outlined in i) that reacts with 2,4 dinitrophenyl hydrazine. iv) indicate whether the reaction as outlined in i) would generate a 'high' or 'low' 510 nm absorbance reading in the presence of NADP+ or in the presence of NADPH, respectively. Justify your answer in a single sentence. Absorbance 510 nm in presences of NADP+: Absorbance 510 nm in presences of NADPH: Justification:

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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2,4-dinitrophenyl hydrazine reacts with the carbonyl groups of ketone molecules to produce a coloured compound as per practical 3. H H C=0 + H₂N-N- H i) ii) O₂N -NO2 H₂O O₂N R -NO2 Provide the balanced equation of the reaction as it occurs in the pentose phosphate pathway (do not show any structures). Analyze the 6-phosphogluconate dehydrogenase reaction. Name the chemical reaction that the substrate of the reaction is subjected to in the pentose phosphate pathway? iii) Name the intermediate and the functional group of the reaction outlined in i) that reacts with 2,4 dinitrophenyl hydrazine. iv) indicate whether the reaction as outlined in i) would generate a 'high' or 'low' 510 nm absorbance reading in the presence of NADP+ or in the presence of NADPH, respectively. Justify your answer in a single sentence. Absorbance 510 nm in presences of NADP+: Absorbance 510 nm in presences of NADPH: Justification:
Transcribed Image Text:2,4-dinitrophenyl hydrazine reacts with the carbonyl groups of ketone molecules to produce a coloured compound as per practical 3. H H C=0 + H₂N-N- H i) ii) O₂N -NO2 H₂O O₂N R -NO2 Provide the balanced equation of the reaction as it occurs in the pentose phosphate pathway (do not show any structures). Analyze the 6-phosphogluconate dehydrogenase reaction. Name the chemical reaction that the substrate of the reaction is subjected to in the pentose phosphate pathway? iii) Name the intermediate and the functional group of the reaction outlined in i) that reacts with 2,4 dinitrophenyl hydrazine. iv) indicate whether the reaction as outlined in i) would generate a 'high' or 'low' 510 nm absorbance reading in the presence of NADP+ or in the presence of NADPH, respectively. Justify your answer in a single sentence. Absorbance 510 nm in presences of NADP+: Absorbance 510 nm in presences of NADPH: Justification:
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