Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
2,4-dinitrophenyl hydrazine reacts with the carbonyl groups of ketone molecules to produce a coloured compound as per practical 3. H H C=0 + H₂N-N- H i) ii) O₂N -NO2 H₂O O₂N R -NO2 Provide the balanced equation of the reaction as it occurs in the pentose phosphate pathway (do not show any structures). Analyze the 6-phosphogluconate dehydrogenase reaction. Name the chemical reaction that the substrate of the reaction is subjected to in the pentose phosphate pathway? iii) Name the intermediate and the functional group of the reaction outlined in i) that reacts with 2,4 dinitrophenyl hydrazine. iv) indicate whether the reaction as outlined in i) would generate a 'high' or 'low' 510 nm absorbance reading in the presence of NADP+ or in the presence of NADPH, respectively. Justify your answer in a single sentence. Absorbance 510 nm in presences of NADP+: Absorbance 510 nm in presences of NADPH: Justification:

2,4-dinitrophenyl hydrazine reacts with the carbonyl groups of ketone molecules to produce a coloured compound as per practical 3. H H C=0 + H₂N-N- H i) ii) O₂N -NO2 H₂O O₂N R -NO2 Provide the balanced equation of the reaction as it occurs in the pentose phosphate pathway (do not show any structures). Analyze the 6-phosphogluconate dehydrogenase reaction. Name the chemical reaction that the substrate of the reaction is subjected to in the pentose phosphate pathway? iii) Name the intermediate and the functional group of the reaction outlined in i) that reacts with 2,4 dinitrophenyl hydrazine. iv) indicate whether the reaction as outlined in i) would generate a 'high' or 'low' 510 nm absorbance reading in the presence of NADP+ or in the presence of NADPH, respectively. Justify your answer in a single sentence. Absorbance 510 nm in presences of NADP+: Absorbance 510 nm in presences of NADPH: Justification:

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
Please step by step answer and don't use ASI Answer please
2,4-dinitrophenyl hydrazine reacts with the carbonyl groups of ketone molecules to produce a coloured compound as per practical 3. H H C=0 + H₂N-N- H i) ii) O₂N -NO2 H₂O O₂N R -NO2 Provide the balanced equation of the reaction as it occurs in the pentose phosphate pathway (do not show any structures). Analyze the 6-phosphogluconate dehydrogenase reaction. Name the chemical reaction that the substrate of the reaction is subjected to in the pentose phosphate pathway? iii) Name the intermediate and the functional group of the reaction outlined in i) that reacts with 2,4 dinitrophenyl hydrazine. iv) indicate whether the reaction as outlined in i) would generate a 'high' or 'low' 510 nm absorbance reading in the presence of NADP+ or in the presence of NADPH, respectively. Justify your answer in a single sentence. Absorbance 510 nm in presences of NADP+: Absorbance 510 nm in presences of NADPH: Justification:
Transcribed Image Text:2,4-dinitrophenyl hydrazine reacts with the carbonyl groups of ketone molecules to produce a coloured compound as per practical 3. H H C=0 + H₂N-N- H i) ii) O₂N -NO2 H₂O O₂N R -NO2 Provide the balanced equation of the reaction as it occurs in the pentose phosphate pathway (do not show any structures). Analyze the 6-phosphogluconate dehydrogenase reaction. Name the chemical reaction that the substrate of the reaction is subjected to in the pentose phosphate pathway? iii) Name the intermediate and the functional group of the reaction outlined in i) that reacts with 2,4 dinitrophenyl hydrazine. iv) indicate whether the reaction as outlined in i) would generate a 'high' or 'low' 510 nm absorbance reading in the presence of NADP+ or in the presence of NADPH, respectively. Justify your answer in a single sentence. Absorbance 510 nm in presences of NADP+: Absorbance 510 nm in presences of NADPH: Justification:
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS