2,4-Dinitrofluorobenzene, very often known as Sanger's reagent after the English chemist Frederick Sanger who popularized its use, reacts selectively with the N-terminal amino group of a polypeptide chain. Sanger was awarded the 1958 Nobel Prize in Chemistry for his work in determining the primary structure of bovine insulin. One of the few people to be awarded two Nobel Prizes, he also shared the 1980 award in chemistry with American chemists Paul Berg and Walter Gilbert for the development of chemical and biological analyses of DNA. polypeptide chain in which the -F + H,NCHČNHCHC-polypeptide N-terminal amino acid is labeled with a 2,4-dinitrophenyl group O,N- R2 NO, (N-Terminal end of a polypeptide chain) 2,4-Dinitro- fluorobenzene Following reaction with 2,4-dinitrofluorobenzene, all amide bonds of the polypeptide chain are hydrolyzed and the amino acid labeled with a 2,4-dinitrophenyl group is sep- arated by either paper or column chromatography and identified.
2,4-Dinitrofluorobenzene, very often known as Sanger's reagent after the English chemist Frederick Sanger who popularized its use, reacts selectively with the N-terminal amino group of a polypeptide chain. Sanger was awarded the 1958 Nobel Prize in Chemistry for his work in determining the primary structure of bovine insulin. One of the few people to be awarded two Nobel Prizes, he also shared the 1980 award in chemistry with American chemists Paul Berg and Walter Gilbert for the development of chemical and biological analyses of DNA. polypeptide chain in which the -F + H,NCHČNHCHC-polypeptide N-terminal amino acid is labeled with a 2,4-dinitrophenyl group O,N- R2 NO, (N-Terminal end of a polypeptide chain) 2,4-Dinitro- fluorobenzene Following reaction with 2,4-dinitrofluorobenzene, all amide bonds of the polypeptide chain are hydrolyzed and the amino acid labeled with a 2,4-dinitrophenyl group is sep- arated by either paper or column chromatography and identified.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Compare and contrast the structural information that can be obtained from use of Sanger’s reagent with that from use of the Edman degradation.
Transcribed Image Text:2,4-Dinitrofluorobenzene, very often known as Sanger's reagent after the English
chemist Frederick Sanger who popularized its use, reacts selectively with the
N-terminal amino group of a polypeptide chain. Sanger was awarded the 1958 Nobel
Prize in Chemistry for his work in determining the primary structure of bovine insulin.
One of the few people to be awarded two Nobel Prizes, he also shared the 1980 award
in chemistry with American chemists Paul Berg and Walter Gilbert for the development
of chemical and biological analyses of DNA.
polypeptide chain in which the
-F + H,NCHČNHCHC-polypeptide N-terminal amino acid is labeled
with a 2,4-dinitrophenyl group
O,N-
R2
NO,
(N-Terminal end of
a polypeptide chain)
2,4-Dinitro-
fluorobenzene
Following reaction with 2,4-dinitrofluorobenzene, all amide bonds of the polypeptide
chain are hydrolyzed and the amino acid labeled with a 2,4-dinitrophenyl group is sep-
arated by either paper or column chromatography and identified.
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