22.124 Give two reasons why bacterial cells are used for recombinant DNA procedures. 22.125 What role do plasmids play in recombinant DNA procedures? 22.126 Describe what occurs when a particular restric- tion enzyme operates on a segment of double- stranded DNA.

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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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22.124 Give two reasons why bacterial cells am wred
for recombinant DNA procedures.
Nucleic Acids
1015
22.125 What role do plasmids play in recombinant
Polymerase Chain Reaction (Section 22.15)
22.131 What is the function of the polymerase chain
DNA procedures?
22.126 Describe what occurs when a particular restric-
reaction?
tion enzyme operates on a segment of double-
stranded DNA.
22.127 Describe what happens during transformation.
22.128 How are plasmids obtained from E, coli bacte-
22.132 What is the function of the enzyme DNA
polymerase in the PCR process?
22.133 What is a primer and what is its function in the
PCR process?
22.134 What are the four types of substances needed
to carry out the PCR process?
ria?
22.129 A particular restriction enzyme will cleave
DNA Sequencing (Section 22.16)
DNA between A and A in the sequence
AAGCTT in the 5'-to-3' direction. Draw a dia-
gram showing the structural details of the
"sticky ends" that result from cleavage of the
following DNA segment.
22.135 How do the notations DATP and ddATP differ
in meaning?
22.136 What role do dideoxynucleotides play in the
DNA sequencing process?
22.137 Assume that the red lines in the first and sec-
ond columns of Figure 22.31d are inter-
changed, while the other labels stay the same.
a. Given this change, what would be the
sequence of bases in the DNA fragment
under study?
b. Given this change, what would be the
sequence of bases in the original DNA
fragment that was to be sequenced?
5'
C CAA GCT
G G T.T C GA
3'
A C
5'
3'
22.130 A particular restriction enzyme will cleave
DNA between A and A in the sequence
AAGCTT in the 5'-to-3' direction. Draw a dia-
22.138 Assame that the "red lines" in the second and
fourth columns of Figure 22.31d are inter-
changed, while the other labels stay the same.
a. Given this change, what would be the
sequence of bases in the DNA fragment
under study?
b. Given this change, what would be the
sequence of bases in the original DNA frag-
ment that was to be sequenced?
gram showing the structural details of the
"sticky ends" that result from cleavage of the
following DNA segment.
3'
5'
G GAA GCT TA
ССТТ CGAAТ
Transcribed Image Text:22.124 Give two reasons why bacterial cells am wred for recombinant DNA procedures. Nucleic Acids 1015 22.125 What role do plasmids play in recombinant Polymerase Chain Reaction (Section 22.15) 22.131 What is the function of the polymerase chain DNA procedures? 22.126 Describe what occurs when a particular restric- reaction? tion enzyme operates on a segment of double- stranded DNA. 22.127 Describe what happens during transformation. 22.128 How are plasmids obtained from E, coli bacte- 22.132 What is the function of the enzyme DNA polymerase in the PCR process? 22.133 What is a primer and what is its function in the PCR process? 22.134 What are the four types of substances needed to carry out the PCR process? ria? 22.129 A particular restriction enzyme will cleave DNA Sequencing (Section 22.16) DNA between A and A in the sequence AAGCTT in the 5'-to-3' direction. Draw a dia- gram showing the structural details of the "sticky ends" that result from cleavage of the following DNA segment. 22.135 How do the notations DATP and ddATP differ in meaning? 22.136 What role do dideoxynucleotides play in the DNA sequencing process? 22.137 Assume that the red lines in the first and sec- ond columns of Figure 22.31d are inter- changed, while the other labels stay the same. a. Given this change, what would be the sequence of bases in the DNA fragment under study? b. Given this change, what would be the sequence of bases in the original DNA fragment that was to be sequenced? 5' C CAA GCT G G T.T C GA 3' A C 5' 3' 22.130 A particular restriction enzyme will cleave DNA between A and A in the sequence AAGCTT in the 5'-to-3' direction. Draw a dia- 22.138 Assame that the "red lines" in the second and fourth columns of Figure 22.31d are inter- changed, while the other labels stay the same. a. Given this change, what would be the sequence of bases in the DNA fragment under study? b. Given this change, what would be the sequence of bases in the original DNA frag- ment that was to be sequenced? gram showing the structural details of the "sticky ends" that result from cleavage of the following DNA segment. 3' 5' G GAA GCT TA ССТТ CGAAТ
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