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22. The membrane potential difference (Ay or Vm) across the inner mitochondrial membrane is -180 mV and the hydrogen concentration in the matrix is 10-7.7 M and the hydrogen concentration in the cytoplasm (intermembrane space) is 107.3 M. The temperature is 25°C. a. What is the pH of the i. matrix ii. cytoplasm b. Calculate the free energy liberated (AG) when protons move down the electrochemical gradient (note this is the same as the proton electrochemical gradient expressed in kcal/mol).

22. The membrane potential difference (Ay or Vm) across the inner mitochondrial membrane is -180 mV and the hydrogen concentration in the matrix is 10-7.7 M and the hydrogen concentration in the cytoplasm (intermembrane space) is 107.3 M. The temperature is 25°C. a. What is the pH of the i. matrix ii. cytoplasm b. Calculate the free energy liberated (AG) when protons move down the electrochemical gradient (note this is the same as the proton electrochemical gradient expressed in kcal/mol).

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Parts A and B
22. The membrane potential difference (Ay or Vm) across the inner mitochondrial membrane is -180 mV and the hydrogen concentration in the matrix is 107.7 M and the hydrogen concentration in the cytoplasm (intermembrane space) is 1073 M. The temperature is 25°C. a. What is the pH of the i. matrix ii. cytoplasm b. Calculate the free energy liberated (AG) when protons move down the electrochemical gradient (note this is the same as the proton electrochemical gradient expressed in kcal/mol).
Transcribed Image Text:22. The membrane potential difference (Ay or Vm) across the inner mitochondrial membrane is -180 mV and the hydrogen concentration in the matrix is 107.7 M and the hydrogen concentration in the cytoplasm (intermembrane space) is 1073 M. The temperature is 25°C. a. What is the pH of the i. matrix ii. cytoplasm b. Calculate the free energy liberated (AG) when protons move down the electrochemical gradient (note this is the same as the proton electrochemical gradient expressed in kcal/mol).
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