2. Which enzyme concentration produced the greatest concentration of mono and disaccharides after 10 minutes? Explain how your Benedict's test results support your answer.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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See attached, you have a table that is perhaps required for some of the questions. 
Answer question number 2 in your own words. Do not copy and paste from google

Lab 5: Enzymes
Results:
Table 1, Effect of enzyme concentration
Amount of lodine Test Results (color)
Amylase
Enzyme
0 drops
3 drops
6 drops
9 drops
Boiling water
bath
37° water
bath
Ice
bath
0 min
water
dark blue
Table 2. Effect of temperature
Temperature
dark blue
dark blue
dark blue
lodine Test
Results
(color)
10 min
dark blue
yellow
Brown
5 min
dark blue
dark brown
brown
light brown
5
6
7
10 min
9
dark blue
Table 3. Effect of pH
pH
light brown
light green
green
5 min
yellow
yellow
yellow
Benedict's
Test Results
(color)
lodine Test
Results (color)
yellow
10 min
bright blue
light blue
yellow
yellow/red
lodine Test
Results (color)
15 min
yellow
yellow
yellow
faint yellow
Transcribed Image Text:Lab 5: Enzymes Results: Table 1, Effect of enzyme concentration Amount of lodine Test Results (color) Amylase Enzyme 0 drops 3 drops 6 drops 9 drops Boiling water bath 37° water bath Ice bath 0 min water dark blue Table 2. Effect of temperature Temperature dark blue dark blue dark blue lodine Test Results (color) 10 min dark blue yellow Brown 5 min dark blue dark brown brown light brown 5 6 7 10 min 9 dark blue Table 3. Effect of pH pH light brown light green green 5 min yellow yellow yellow Benedict's Test Results (color) lodine Test Results (color) yellow 10 min bright blue light blue yellow yellow/red lodine Test Results (color) 15 min yellow yellow yellow faint yellow
Part 2
1. At which enzyme concentration was starch hydrolyzed the fastest? Slowest?
Explain how your iodine test results support your answer.
2. Which enzyme concentration produced the greatest concentration of mono and
disaccharides after 10 minutes? Explain how your Benedict's test results
support your answer.
3. If you had conducted Benedict's test on the samples at time 0 minutes, what
results would you expect to obtain for each test tube?
4. How would you summarize the effect of enzyme concentration on enzyme
activity?
Transcribed Image Text:Part 2 1. At which enzyme concentration was starch hydrolyzed the fastest? Slowest? Explain how your iodine test results support your answer. 2. Which enzyme concentration produced the greatest concentration of mono and disaccharides after 10 minutes? Explain how your Benedict's test results support your answer. 3. If you had conducted Benedict's test on the samples at time 0 minutes, what results would you expect to obtain for each test tube? 4. How would you summarize the effect of enzyme concentration on enzyme activity?
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