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2. Consider the following TLC plate depictions below. In each depiction, 2 unknown samples are spotted side by side. For each depiction, (a) determine the error, if any, and (b) propose a modification

2. Consider the following TLC plate depictions below. In each depiction, 2 unknown samples are spotted side by side. For each depiction, (a) determine the error, if any, and (b) propose a modification

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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3a. 3b

### Thin Layer Chromatography (TLC) Plate Analysis **Problem Statement:** Consider the following TLC plate depictions below. In each depiction, 2 unknown samples are spotted side by side. For each depiction, (a) determine the error, if any, and (b) propose a modification. ### Plate Depictions: There are three TLC plates labeled Plate I, Plate II, and Plate III, each showcasing errors in technique or procedure and improvements needed. #### **Plate I:** - **Error:** The spots are too large and not well-defined - **Modification:** Apply smaller, more concentrated spots of the unknown samples to ensure clearer separation of components. #### **Plate II:** - **Error:** The spots are overlapping and conflated, causing unclear results. - **Modification:** Spot the samples with greater care and allow sufficient space between them. It also may help to apply the samples lightly in multiple small taps rather than a single large application. #### **Plate III:** - **Observation:** - Sample A shows two distinct spots indicating the presence of 2 compounds: Compound 1 and Compound 2. - Sample B shows one spot corresponding to Compound 2. - **Conclusion:** As there are no notable errors, this plate correctly identifies and separates the compounds. **Error Analysis:** For Plates I and II, the incorrect technique of sample application results in poor separation and uninformative spots. Correct spot size, concentration, and careful application technique significantly improve the results. **Diagram Explanation:** - In each TLC plate image, the baseline (where samples are initially spotted) is denoted by a dashed horizontal line at the bottom. - The solvent front (the furthest point reached by the solvent) is indicated by a dashed horizontal line near the top of each plate. - Compounds are seen as spots that travel up the plate based on their affinity to the solvent and stationary phase. ### Educational Takeaway: Proper technique in spotting samples on a TLC plate is crucial for effective separation and accurate identification of compounds. Ensuring appropriate spot size, careful application, and adequate spacing between the samples will yield better chromatographic results. ### References: For more details on Thin Layer Chromatography (TLC) and best practices for sample application, refer to [recommended resources or textbooks].
Transcribed Image Text:### Thin Layer Chromatography (TLC) Plate Analysis **Problem Statement:** Consider the following TLC plate depictions below. In each depiction, 2 unknown samples are spotted side by side. For each depiction, (a) determine the error, if any, and (b) propose a modification. ### Plate Depictions: There are three TLC plates labeled Plate I, Plate II, and Plate III, each showcasing errors in technique or procedure and improvements needed. #### **Plate I:** - **Error:** The spots are too large and not well-defined - **Modification:** Apply smaller, more concentrated spots of the unknown samples to ensure clearer separation of components. #### **Plate II:** - **Error:** The spots are overlapping and conflated, causing unclear results. - **Modification:** Spot the samples with greater care and allow sufficient space between them. It also may help to apply the samples lightly in multiple small taps rather than a single large application. #### **Plate III:** - **Observation:** - Sample A shows two distinct spots indicating the presence of 2 compounds: Compound 1 and Compound 2. - Sample B shows one spot corresponding to Compound 2. - **Conclusion:** As there are no notable errors, this plate correctly identifies and separates the compounds. **Error Analysis:** For Plates I and II, the incorrect technique of sample application results in poor separation and uninformative spots. Correct spot size, concentration, and careful application technique significantly improve the results. **Diagram Explanation:** - In each TLC plate image, the baseline (where samples are initially spotted) is denoted by a dashed horizontal line at the bottom. - The solvent front (the furthest point reached by the solvent) is indicated by a dashed horizontal line near the top of each plate. - Compounds are seen as spots that travel up the plate based on their affinity to the solvent and stationary phase. ### Educational Takeaway: Proper technique in spotting samples on a TLC plate is crucial for effective separation and accurate identification of compounds. Ensuring appropriate spot size, careful application, and adequate spacing between the samples will yield better chromatographic results. ### References: For more details on Thin Layer Chromatography (TLC) and best practices for sample application, refer to [recommended resources or textbooks].
Transcription: --- **III(a):** **III(b):** --- Explanation for missing content: The image provided contains only the subheadings "III(a):" and "III(b):" with no additional text, graphs, or diagrams listed beneath these headings. These subheadings likely suggest subsections that require additional information or content to be filled in. Please provide any further text or context to be included under these headings, or let us know if there are any specific topics or details that should be expanded upon for educational purposes.
Transcribed Image Text:Transcription: --- **III(a):** **III(b):** --- Explanation for missing content: The image provided contains only the subheadings "III(a):" and "III(b):" with no additional text, graphs, or diagrams listed beneath these headings. These subheadings likely suggest subsections that require additional information or content to be filled in. Please provide any further text or context to be included under these headings, or let us know if there are any specific topics or details that should be expanded upon for educational purposes.
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