Homework Help is Here – Start Your Trial Now!
Science
Chemistry
2. Consider the following TLC plate depictions below. In each depiction, 2 unknown samples are spotted side by side. For each depiction, (a) determine the error, if any, and (b) propose a modification

2. Consider the following TLC plate depictions below. In each depiction, 2 unknown samples are spotted side by side. For each depiction, (a) determine the error, if any, and (b) propose a modification

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
See similar textbooks
icon
Related questions
Question

3a. 3b

### Thin Layer Chromatography (TLC) Plate Analysis **Problem Statement:** Consider the following TLC plate depictions below. In each depiction, 2 unknown samples are spotted side by side. For each depiction, (a) determine the error, if any, and (b) propose a modification. ### Plate Depictions: There are three TLC plates labeled Plate I, Plate II, and Plate III, each showcasing errors in technique or procedure and improvements needed. #### **Plate I:** - **Error:** The spots are too large and not well-defined - **Modification:** Apply smaller, more concentrated spots of the unknown samples to ensure clearer separation of components. #### **Plate II:** - **Error:** The spots are overlapping and conflated, causing unclear results. - **Modification:** Spot the samples with greater care and allow sufficient space between them. It also may help to apply the samples lightly in multiple small taps rather than a single large application. #### **Plate III:** - **Observation:** - Sample A shows two distinct spots indicating the presence of 2 compounds: Compound 1 and Compound 2. - Sample B shows one spot corresponding to Compound 2. - **Conclusion:** As there are no notable errors, this plate correctly identifies and separates the compounds. **Error Analysis:** For Plates I and II, the incorrect technique of sample application results in poor separation and uninformative spots. Correct spot size, concentration, and careful application technique significantly improve the results. **Diagram Explanation:** - In each TLC plate image, the baseline (where samples are initially spotted) is denoted by a dashed horizontal line at the bottom. - The solvent front (the furthest point reached by the solvent) is indicated by a dashed horizontal line near the top of each plate. - Compounds are seen as spots that travel up the plate based on their affinity to the solvent and stationary phase. ### Educational Takeaway: Proper technique in spotting samples on a TLC plate is crucial for effective separation and accurate identification of compounds. Ensuring appropriate spot size, careful application, and adequate spacing between the samples will yield better chromatographic results. ### References: For more details on Thin Layer Chromatography (TLC) and best practices for sample application, refer to [recommended resources or textbooks].
Transcribed Image Text:### Thin Layer Chromatography (TLC) Plate Analysis **Problem Statement:** Consider the following TLC plate depictions below. In each depiction, 2 unknown samples are spotted side by side. For each depiction, (a) determine the error, if any, and (b) propose a modification. ### Plate Depictions: There are three TLC plates labeled Plate I, Plate II, and Plate III, each showcasing errors in technique or procedure and improvements needed. #### **Plate I:** - **Error:** The spots are too large and not well-defined - **Modification:** Apply smaller, more concentrated spots of the unknown samples to ensure clearer separation of components. #### **Plate II:** - **Error:** The spots are overlapping and conflated, causing unclear results. - **Modification:** Spot the samples with greater care and allow sufficient space between them. It also may help to apply the samples lightly in multiple small taps rather than a single large application. #### **Plate III:** - **Observation:** - Sample A shows two distinct spots indicating the presence of 2 compounds: Compound 1 and Compound 2. - Sample B shows one spot corresponding to Compound 2. - **Conclusion:** As there are no notable errors, this plate correctly identifies and separates the compounds. **Error Analysis:** For Plates I and II, the incorrect technique of sample application results in poor separation and uninformative spots. Correct spot size, concentration, and careful application technique significantly improve the results. **Diagram Explanation:** - In each TLC plate image, the baseline (where samples are initially spotted) is denoted by a dashed horizontal line at the bottom. - The solvent front (the furthest point reached by the solvent) is indicated by a dashed horizontal line near the top of each plate. - Compounds are seen as spots that travel up the plate based on their affinity to the solvent and stationary phase. ### Educational Takeaway: Proper technique in spotting samples on a TLC plate is crucial for effective separation and accurate identification of compounds. Ensuring appropriate spot size, careful application, and adequate spacing between the samples will yield better chromatographic results. ### References: For more details on Thin Layer Chromatography (TLC) and best practices for sample application, refer to [recommended resources or textbooks].
Transcription: --- **III(a):** **III(b):** --- Explanation for missing content: The image provided contains only the subheadings "III(a):" and "III(b):" with no additional text, graphs, or diagrams listed beneath these headings. These subheadings likely suggest subsections that require additional information or content to be filled in. Please provide any further text or context to be included under these headings, or let us know if there are any specific topics or details that should be expanded upon for educational purposes.
Transcribed Image Text:Transcription: --- **III(a):** **III(b):** --- Explanation for missing content: The image provided contains only the subheadings "III(a):" and "III(b):" with no additional text, graphs, or diagrams listed beneath these headings. These subheadings likely suggest subsections that require additional information or content to be filled in. Please provide any further text or context to be included under these headings, or let us know if there are any specific topics or details that should be expanded upon for educational purposes.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Quality Assurance and Calibration Methods
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
SEE MORE TEXTBOOKS