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- In basic spectrophotometer, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2compute for the callibration factor in micrometers of the ocular micrometer using the low power objective. explain your answerFOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?
- in basic spectrophotometer technique, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 CM-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2.2. Compute for the calibration factor. Show your solution. CF = stage micrometer divisions subtended by om x value of one stage µm division ocular micrometer divisions subtended by stage micrometer (1 su x 10μm) 10 ou CF = CF = 1μm Ocular scale Stage micrometer scale or CF = Computation: (stage units x 10μm) ocular units 60 70 80 90 100 Name of Organism: Organism A Objective Used: HPO Total Magnification: 450x Ocular Micrometer units (violet rods): 10 Length of Organism:Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…
- From the basic spectrophotometer technique, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of light the beam transmitted through a 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 25mg/mL. Compare between 1 and 2.Solve for the calibration factor in micrometers of the ocular micrometer using the LPO5. n° of 2-chloro-2-methylpropane is 1.3850. If a 1g sample of 2-chloro-2-methylpropane was contaminated with 1 drop of ethanol, what do you think the effect would be on the refractive index and by approximately how much? (1 drop of ethanol weighs about 0.03 g).
- Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the distance of one ocular division? Show your solution.In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.1. Calculate the total magnification for the following: A. Scanning power objective = 5x; eyepiece =4x B. High power objective = 40x; eyepiece= 5x c. Low power objective = 10x; eyepiece= 10x