2 One of the common methods used to determine phosphorus in urine isto treat urine with molybdenum(VI) resulting in a complex compoundof 12- molybdophosphate and then reduced using ascorbic acid produces a blue-colored compound called molybdenum blue (max = 650 nm). A patient's urine sample collected over 24 hours produced 1122 ml. of urine. A total of 1 mL of the sample was treated with Mo(VI) and ascorbic acid and diluted until it reached a volume of 50 mL. A calibration curve is also prepared by treating 1 mL of standard phosphate solution in the same way. The absorbance of the standard solution and the sample was then measured at a wavelength of 650 nm and produced the following data: Solution Absorbance at λ 650 nm Phosphate 1 ppm Phosphate 2 ppm Phosphate 3 ppm Phosphate 4 ppm Urine sample 0,230 0,436 0,638 0,848 0,518 a. Create a calibration curve for the data and determine the slope, intercept, and R values of 2! Determine the concentration of phosphates found in urine samples in units of ppm and mM! b. Determine the mass of phosphate excreted by the patient for 24 hours?

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
icon
Related questions
Question
2. One of the common methods used to determine phosphorus in urine isto treat urine with
molybdenum(VI) resulting in a complex compoundof 12-
molybdophosphate and then reduced using ascorbic acid produces a blue-colored
compound called molybdenum blue (max = 650 nm). A patient's urine sample collected
over 24 hours produced 1122 mL of urine. A total of 1 mL of the sample was treated with
Mo(VI) and ascorbic acid and diluted until it reached a volume of 50 mL. A calibration
curve is also prepared by treating 1 mL of standard phosphate solution in the same
way. The absorbance of the standard solution and the sample was then measured at a
wavelength of 650 nm and produced the following data:
Solution
Absorbance at λ 650 nm
Phosphate 1 ppm
Phosphate 2 ppm
Phosphate 3 ppm
Phosphate 4 ppm
Urine sample
0,230
0.436
0,638
0,848
0.518
a. Create a calibration curve for the data and determine the slope, intercept, and R values
of 2! Determine the concentration of phosphates found in urine samples in units of
ppm and mM!
b. Determine the mass of phosphate excreted by the patient for 24 hours?
Transcribed Image Text:2. One of the common methods used to determine phosphorus in urine isto treat urine with molybdenum(VI) resulting in a complex compoundof 12- molybdophosphate and then reduced using ascorbic acid produces a blue-colored compound called molybdenum blue (max = 650 nm). A patient's urine sample collected over 24 hours produced 1122 mL of urine. A total of 1 mL of the sample was treated with Mo(VI) and ascorbic acid and diluted until it reached a volume of 50 mL. A calibration curve is also prepared by treating 1 mL of standard phosphate solution in the same way. The absorbance of the standard solution and the sample was then measured at a wavelength of 650 nm and produced the following data: Solution Absorbance at λ 650 nm Phosphate 1 ppm Phosphate 2 ppm Phosphate 3 ppm Phosphate 4 ppm Urine sample 0,230 0.436 0,638 0,848 0.518 a. Create a calibration curve for the data and determine the slope, intercept, and R values of 2! Determine the concentration of phosphates found in urine samples in units of ppm and mM! b. Determine the mass of phosphate excreted by the patient for 24 hours?
Expert Solution
steps

Step by step

Solved in 5 steps with 2 images

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Introduction to Chemical Engineering Thermodynami…
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Process Dynamics and Control, 4e
Process Dynamics and Control, 4e
Chemical Engineering
ISBN:
9781119285915
Author:
Seborg
Publisher:
WILEY
Industrial Plastics: Theory and Applications
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
Unit Operations of Chemical Engineering
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The