2) A protein is to be salted out of solution using ammonium sulfate. The protein solution is initially at 20 g/L. A plot of soluble protein vs. ammonium sulfate concentration is given below. a) What are the Cohn equation coefficients? b) Calculate the concentration of ammonium sulfate to precipitate out 99% of the protein. 4 2 y= -2.63x+7.54 R² = 1 In(S) 0 2 4 6 -2 -4 [Ammonium sulfate] (mol/L)

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
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2) A protein is to be salted out of solution using ammonium sulfate. The protein solution is
initially at 20 g/L. A plot of soluble protein vs. ammonium sulfate concentration is given below.
a) What are the Cohn equation coefficients?
b) Calculate the concentration of ammonium sulfate
to precipitate out 99% of the protein.
4
2
y= -2.63x+7.54
R² = 1
In(S)
0
2
4
6
-2
-4
[Ammonium sulfate] (mol/L)
Transcribed Image Text:2) A protein is to be salted out of solution using ammonium sulfate. The protein solution is initially at 20 g/L. A plot of soluble protein vs. ammonium sulfate concentration is given below. a) What are the Cohn equation coefficients? b) Calculate the concentration of ammonium sulfate to precipitate out 99% of the protein. 4 2 y= -2.63x+7.54 R² = 1 In(S) 0 2 4 6 -2 -4 [Ammonium sulfate] (mol/L)
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