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175ml of 1X SDS buffer. You are provided with 500ml of 6X SDS buffer. How much water and how much of the 6X SDS buffer solution do you have to use to make the desired 1X SDS buffer?

175ml of 1X SDS buffer. You are provided with 500ml of 6X SDS buffer. How much water and how much of the 6X SDS buffer solution do you have to use to make the desired 1X SDS buffer?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Please solve for E and please show all calculations
● 100ml of LB Amp stock at 100 mg/ml Kan stock at 50 mg/ml Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration. d) 50 ml of a solution containing each of the following: 10% Acrylamide, 0.05% BIS, 0.1M Tris-HCl pH 8.9. You have 0.5L of each of the following: 40% (w/v) Acrylamide I 2% (v/v) Bis 2M Tris-HCl pH 8.9 Determine how much you need of each stock solution and dH₂O to prepare desired 50ml mix. e) 175ml of 1X SDS buffer. You are provided with 500ml of 6X SDS buffer. How much water and how much of the 6X SDS buffer solution do you have to use to make the desired 1X SDS buffer? f) 6L of 1mM IPTG. You need to prepare a stock solution of 1M IPTG that will be sufficient to give you 6L of 1mM IPTG once it is diluted. How much water and how much IPTG (in grams) will you need to make a sufficient 1M stock solution?
Transcribed Image Text:● 100ml of LB Amp stock at 100 mg/ml Kan stock at 50 mg/ml Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration. d) 50 ml of a solution containing each of the following: 10% Acrylamide, 0.05% BIS, 0.1M Tris-HCl pH 8.9. You have 0.5L of each of the following: 40% (w/v) Acrylamide I 2% (v/v) Bis 2M Tris-HCl pH 8.9 Determine how much you need of each stock solution and dH₂O to prepare desired 50ml mix. e) 175ml of 1X SDS buffer. You are provided with 500ml of 6X SDS buffer. How much water and how much of the 6X SDS buffer solution do you have to use to make the desired 1X SDS buffer? f) 6L of 1mM IPTG. You need to prepare a stock solution of 1M IPTG that will be sufficient to give you 6L of 1mM IPTG once it is diluted. How much water and how much IPTG (in grams) will you need to make a sufficient 1M stock solution?
Expert Solution
Step 1

To calculate the volume of 6X SDS needed , we would use dilution law. 

Then amount of water would be calculated by subtracting the amount of 6X SDA needed from the total volume of 1X SDS to be made. 

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