13. A 9.50 μM standard solution of X yields an absorbance of 0.657 at 630 nm using a 1.00-cm cuvette. The molar absorptivity for X at 630 nm is a) 40,700 M-¹cm¹ b) 614,000 M-¹cm-1 c) 1010 M-¹cm¹ d) 69,200 M¹¹cm-¹ e) 138,700 M-¹cm¹
13. A 9.50 μM standard solution of X yields an absorbance of 0.657 at 630 nm using a 1.00-cm cuvette. The molar absorptivity for X at 630 nm is a) 40,700 M-¹cm¹ b) 614,000 M-¹cm-1 c) 1010 M-¹cm¹ d) 69,200 M¹¹cm-¹ e) 138,700 M-¹cm¹
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:13. A 9.50 μM standard solution of X yields an absorbance
of 0.657 at 630 nm using a 1.00-cm cuvette. The
molar absorptivity for X at 630 nm is
a) 40,700 M-¹cm-1
b) 614,000 M-¹cm-¹
c) 1010 M-¹cm-1
d) 69,200 M¹¹cm ¹
e) 138,700 M¹¹cm¹
14. The molar absorptivity for ferricyanide ion (FW =
211.95 g/mol) at 420 nm is 1010 M-¹cm¹. What is the
concentration of ferricyanide in a solution that yields
an absorbance of 0.354 in a 1.00-cm cuvette?
a) 30 ppm
b) 45 ppm
c) 60 ppm
d) 75 ppm
e) 90 ppm
15. Deviations from Beer's law are observed when
measurements are made
a) with highly concentrated solutions
b) using polychromatic light sources
c) in the presence of stray light
d) all of these
e) none of these
16. Photometric assays typically involve measuring
absorbance at an analyte's "Amax" in order to
a) maximize sensitivity
b) maximize selectivity
c) maximize accuracy
d) maximize precision
e) maximize detection limit
17. The calibration curves shown at the right indicate the
photometric measurements satisfy Beer's law at both
550 nm and 635 nm. Which statement below is true?
a) analysis at 550 nm yields a more sensitive analysis
b) analysis at 635 nm yields a more sensitive analysis
c) analysis at 550 nm yields a more sel
analysis
d) analysis at 635 nm yields a more selective analysis
e) these curves do NOT satisfy Beer's law
18. A sample solution yielded an absorbance of 0.723 at
550 nm when analyzed using the procedure that
produced the calibration curves at the right. The
concentration of analyte in the sample solution is
a) 15 µg/mL
b) 17 µg/mL
c) 20 µg/mL
d) 23 µg/mL
e) 27 µg/mL
Absorbance
1.2
1
0.8
0.6
0.4
0.2
о
10
20
550 nm
y=0.0405x+0.0191
R² = 0.9989
Concentration (µg/mL)
635 nm
y= 0.0266x+0.0065
R² = 0.999
30
40
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