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10ml of 20% (w/v) L-arabinose stock (weight/volume). How much L-arabinose do you need to prepare desired arabinose stock?

10ml of 20% (w/v) L-arabinose stock (weight/volume). How much L-arabinose do you need to prepare desired arabinose stock?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Please solve for B and please show all calculations
Address the questions regarding the preparation of the following 6 solutions. Show all of your calculations. a) 500ml of 2M Tris-HCI buffer pH 8.9. Tris base MW is 121.14 g/mol. You will use concentrated HCI to adjust the pH. Describe the preparation procedure for this buffer and list the materials needed. b) 10ml of 20% (w/v) L-arabinose stock (weight/volume). How much L-arabinose do you need to prepare desired arabinose stock? c) 100ml of LB media with: 25 µg/ml of Amp; and 100 µg/ml of Kan - final concentrations. You have: ● ● 100ml of LB Amp stock at 100 mg/ml Kan stock at 50 mg/ml ● Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration. d) 50 ml of a solution containing each of the following: 10% Acrylamide, 0.05% BIS, 0.1M Tris-HCl pH 8.9. You have 0.5L of each of the following: 40% (w/v) Acrylamide
Transcribed Image Text:Address the questions regarding the preparation of the following 6 solutions. Show all of your calculations. a) 500ml of 2M Tris-HCI buffer pH 8.9. Tris base MW is 121.14 g/mol. You will use concentrated HCI to adjust the pH. Describe the preparation procedure for this buffer and list the materials needed. b) 10ml of 20% (w/v) L-arabinose stock (weight/volume). How much L-arabinose do you need to prepare desired arabinose stock? c) 100ml of LB media with: 25 µg/ml of Amp; and 100 µg/ml of Kan - final concentrations. You have: ● ● 100ml of LB Amp stock at 100 mg/ml Kan stock at 50 mg/ml ● Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration. d) 50 ml of a solution containing each of the following: 10% Acrylamide, 0.05% BIS, 0.1M Tris-HCl pH 8.9. You have 0.5L of each of the following: 40% (w/v) Acrylamide
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