109 108 Wild Type tlc1 107 - tlc1 rad59 106 - tlc1 rad50 rad59 - tlc1 rad51 rad59 105 • tlc1 rad50 rad51 104 - tlc1 rad52 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Days in culture FIG. 1. RAD59 is required to generate survivors in a rad51 background. Cells were grown to saturation in YPD medium and then diluted to 10° cells/ml every 24 h with fresh ÝPD medium. Cells were counted every 24 h with a hemocytometer. The curves shown are the average of results for four independent clones from each genotype. Cell Density (cells/ml)

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Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.