10. You're tasked with making 100 mL of 50 mM acetate buffer with a pH of 5. You have a solution 0.3 M acetic acid and sodium acetate powder (82 g/mol) available to you. 1. Hoping to avoid tedious titration, what volume of the acetic acid solution and what mass of sodium acetate powder could you combine to theoretically hit pH 5 exactly in ultra-pure water? Assume the acetic acid solution is 100% protonated. (Hint: the combined molar concentration of the acid and conjugate base should be 50 mM.) 2. A lab mate used the last of the sodium acetate, leaving you with only the acetic acid solution. How could you prepare the requested buffer anyway, assuming you have HCI and NaOH solutions to adjust the pH by titration?

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You're tasked with making 100 mL of 50 mM acetate buffer with a pH of 5. You have a solution 0.3 M acetic acid and sodium acetate powder (82 g/mol) available to you.

1. Hoping to avoid tedious titration, what volume of the acetic acid solution and what mass of sodium acetate powder could you combine to theoretically hit pH 5 exactly in ultra-pure water? Assume the acetic acid solution is 100% protonated. (Hint: the combined molar concentration of the acid and conjugate base should be 50 mM.)

2. A lab mate used the last of the sodium acetate, leaving you with only the acetic acid solution. How could you prepare the requested buffer anyway, assuming you have HCl and NaOH solutions to adjust the pH by titration?
Transcribed Image Text:You're tasked with making 100 mL of 50 mM acetate buffer with a pH of 5. You have a solution 0.3 M acetic acid and sodium acetate powder (82 g/mol) available to you. 1. Hoping to avoid tedious titration, what volume of the acetic acid solution and what mass of sodium acetate powder could you combine to theoretically hit pH 5 exactly in ultra-pure water? Assume the acetic acid solution is 100% protonated. (Hint: the combined molar concentration of the acid and conjugate base should be 50 mM.) 2. A lab mate used the last of the sodium acetate, leaving you with only the acetic acid solution. How could you prepare the requested buffer anyway, assuming you have HCl and NaOH solutions to adjust the pH by titration?
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