Buffer is 200 ml of 0.15 M Tris-HCl buffer with a pH of 8.9  a. Calculate the concentration of the conjugate acid and conjugate base in this buffer. (hint: the total concentration of this buffer is 0.15 M i.e. [acid] + [base] = 0.15 M).  b. What mass of Tris-HCl powder would add to get the concentration of acid that you calculated in part 3a? Remember that the total volume of the buffer is 200 ml.  c. What mass of Tris base powder would you add to get the final concentration of base that you calculated in part 3a? Remember that the total volume of the buffer is 200 ml.

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Buffer is 200 ml of 0.15 M Tris-HCl buffer with a pH of 8.9 

a. Calculate the concentration of the conjugate acid and conjugate base in this buffer. (hint: the total concentration of this buffer is 0.15 M i.e. [acid] + [base] = 0.15 M). 

b. What mass of Tris-HCl powder would add to get the concentration of acid that you calculated in part 3a? Remember that the total volume of the buffer is 200 ml. 

c. What mass of Tris base powder would you add to get the final concentration of base that you calculated in part 3a? Remember that the total volume of the buffer is 200 ml. 

 

 

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