10. Fill up the table and draw the following: a. guard cell in distilled water b. guard cell in 0.5 saline solution c. guard cell in 0.9 saline solution d. guard cell in 1.5 saline solution

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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B. Osmosis
For this part of activity, please refer to the procedures listed below.
1. Strip off a thin layer of the outer epidermis of the undersurface of the leaf of
Rhoeo discolor.
2. Place a drop of distilled water on a microscope slide.
3. Add a cover slip and examine under the LPO, then HPO.
4. Focus on the stomata and observe the condition of the guard cells.
5. Apply a small piece of blotting or tissue paper on the edge of the cover slip to
remove the water.
6. Place a drop of 0.5 saline solution on the slide, close to but not on the cover
slip.
7. Draw the solution out under the cover slip by applying again a small piece of
tissue paper to the opposite edge.
8. Examine under the HPO and observe the changes in the guard cells.
9. Repeat procedure numbers 6 to 8, but this time, use the 0.9 saline solution,
after which 1.5 saline solution follows.
10. Fill up the table and draw the following:
a. guard cell in distilled water
b. guard cell in 0.5 saline solution
c. guard cell in 0.9 saline solution
d. guard cell in 1.5 saline solution
Transcribed Image Text:B. Osmosis For this part of activity, please refer to the procedures listed below. 1. Strip off a thin layer of the outer epidermis of the undersurface of the leaf of Rhoeo discolor. 2. Place a drop of distilled water on a microscope slide. 3. Add a cover slip and examine under the LPO, then HPO. 4. Focus on the stomata and observe the condition of the guard cells. 5. Apply a small piece of blotting or tissue paper on the edge of the cover slip to remove the water. 6. Place a drop of 0.5 saline solution on the slide, close to but not on the cover slip. 7. Draw the solution out under the cover slip by applying again a small piece of tissue paper to the opposite edge. 8. Examine under the HPO and observe the changes in the guard cells. 9. Repeat procedure numbers 6 to 8, but this time, use the 0.9 saline solution, after which 1.5 saline solution follows. 10. Fill up the table and draw the following: a. guard cell in distilled water b. guard cell in 0.5 saline solution c. guard cell in 0.9 saline solution d. guard cell in 1.5 saline solution
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