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1. Which medium would you consider to be "complex" and which "defined"? Which is “rich" and which is “minimal"? Explain your answers.

1. Which medium would you consider to be "complex" and which "defined"? Which is “rich" and which is “minimal"? Explain your answers.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have found media that support growth of pure cultures of ETPUM in your laboratory. The recipes for these media are shown below: Table 2 Medium #1 Medium #2 5 g yeast extract 10.5 g K HΡΟ4 20 g tryptone extract 4-5 g KH2PO4 I g MgSO4 10 g polyurethane Il H20 0.5 g NaCl 3.6 g glucose Il H20 Growth in these media: Table 3 Growth Medium #1 Medium #2 ETPUM growth-aerobic + ETPUM growth-anaerobic E.coli growth-aerobic + E.coli growth-anaerobic You are excited because, in Medium #2, ETPUM utilizes polyurethane as its energy source and its sole source of carbon and nitrogen, a finding that raises the possibility that ETPUM could be a useful tool for bioremediation of polyurethane-containing wastes (in landfills, etc.). You have also made some progress in characterizing the central metabolic pathways and related biochemical activities of ETPUM. In particular, you have discovered that: • ETPUM secretes an enzyme (polyurethanase) that catalyzes degradation of polyurethane, generating citric acid (citrate) as a product. • The cytoplasmic membrane of ETPUM contains an ABC transport system capable of transporting citrate across the membrane at the expense of 4 ATP molecules (hydrolyzed to form ADP and phosphate) per molecule of citrate transported. • The cytoplasm of this organism contains all of the enzymes required for glycolysis, and for the TCA cycle. • The cytoplasmic membrane of ETPUM contains proteins that form a functional electron-transport pathway (that uses O2 as the terminal electron acceptor).
Transcribed Image Text:You have found media that support growth of pure cultures of ETPUM in your laboratory. The recipes for these media are shown below: Table 2 Medium #1 Medium #2 5 g yeast extract 10.5 g K HΡΟ4 20 g tryptone extract 4-5 g KH2PO4 I g MgSO4 10 g polyurethane Il H20 0.5 g NaCl 3.6 g glucose Il H20 Growth in these media: Table 3 Growth Medium #1 Medium #2 ETPUM growth-aerobic + ETPUM growth-anaerobic E.coli growth-aerobic + E.coli growth-anaerobic You are excited because, in Medium #2, ETPUM utilizes polyurethane as its energy source and its sole source of carbon and nitrogen, a finding that raises the possibility that ETPUM could be a useful tool for bioremediation of polyurethane-containing wastes (in landfills, etc.). You have also made some progress in characterizing the central metabolic pathways and related biochemical activities of ETPUM. In particular, you have discovered that: • ETPUM secretes an enzyme (polyurethanase) that catalyzes degradation of polyurethane, generating citric acid (citrate) as a product. • The cytoplasmic membrane of ETPUM contains an ABC transport system capable of transporting citrate across the membrane at the expense of 4 ATP molecules (hydrolyzed to form ADP and phosphate) per molecule of citrate transported. • The cytoplasm of this organism contains all of the enzymes required for glycolysis, and for the TCA cycle. • The cytoplasmic membrane of ETPUM contains proteins that form a functional electron-transport pathway (that uses O2 as the terminal electron acceptor).
Questions 1. Which medium would you consider to be "complex" and which "defined"? Which is "rich" and which is “minimal"? Explain your answers. 2. Given that polyurethane is a huge polymer (MW >>100,000 Daltons), why is it important that the polyurethanase is a secreted enzyme? If we assume that the polyurethane is the source of energy for the "ELVIS Meltdown!" by Stewart, Smith and Shields Page 7 organism, how can material (carbon atoms) from it find its way into the central metabolic pathways of this microbe? What is the "entry point"? What happens after its entry into the metabolic pathway?
Transcribed Image Text:Questions 1. Which medium would you consider to be "complex" and which "defined"? Which is "rich" and which is “minimal"? Explain your answers. 2. Given that polyurethane is a huge polymer (MW >>100,000 Daltons), why is it important that the polyurethanase is a secreted enzyme? If we assume that the polyurethane is the source of energy for the "ELVIS Meltdown!" by Stewart, Smith and Shields Page 7 organism, how can material (carbon atoms) from it find its way into the central metabolic pathways of this microbe? What is the "entry point"? What happens after its entry into the metabolic pathway?
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