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- Tube Observation Inference 0.1000 M glucose Red precipitate 0.0500 M glucose Red precipitate 0.0250 M glucose Red precipitate 0.0100 M glucose Green precipitate 0.0010 M glucose Green precipitate 0.0001 M glucose Blue solution 0.1000 M lactose Red precipitate 0.1000 M sucrose Blue solution 0.1000 M fructose Red precipitate Urine 1 Green solution Urine 2 Orange precipitate Table 3. Results of Benedicts Test on different samples1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to completely grind the beef? 3. Why is necessary to balance the homogenate tubes for centrifugation? 4. How do you prepare a 100mL of 0.1 M phosphate buffer? 5. From the anterior buffer, how do you make 100mL of 0.05 M? 6. Calculate the amount of ammonium sulfate necessary to get a 20% solution? 7. Which is the importance of dialysis?What is the clinical significance of ESR determination? Differentiate Wintrobe from Westergreen regarding accuracy of the test in a tabulated order. NOTE: Kindly asnwer all the questions. Thank you!
- 1. Give the importance and purpose of the Cetrimide Test. 2. Discuss briefly how each of the different oxidase method is being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.2. What sample shows a positive result with the Molisch Test? Why did this sample give a positive result? 3. What is the chemical basis of the Xanthoproteic Test? 4. Egg white and milk are used as antidotes for heavy metal poisoning. Explain. 5. According to advertisements, commercial shampoo or conditioner contains “protein." Suggest a test that could be done on the product to check this claim? Explain your answer.1) You have been asked to make up four 1.5% agarose gels at 30 ml each. Ethidium Bromide is to be added at 0.5 ug/ml and you have a bottle at 10ug/ml. Write down step-by-step protocol of how to proceed. 2. How would you make up one liter of 1X TAE buffer using a 25X stock?