What is the charge of the primary structure of proteins at pH = 2.0, 4.0,6.0and 11.0 of the following proteins. Draw their structures and predict their net1.charge.A) Lys- Gly- Ala- GlyB) Lys- Gly-Ala-GluC) His- Gly- Ala- GluD) Glu- Gly- Ala -GluE) Gln-Gly-Ala-Lys2. Calculate the pI of the following primary structure of protein3. the migration toward the cathode, the negative pole; A for the migration towardthe anode, the positive pole; and o if the peptide remains stationary.

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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1. Isoelectric point of polypeptide: (1) is pH at which the o-carboxyl and o-amino groups are uncharged. (2) is pH at which the net charge on the molecules in solution is 0. (3) is pH at which the charge of the o-carboxyl group is higher than the charge of the o- amino group. А. (1) only В. (2) only С. (3) only D. (1) and (3) E. (2) and(3)
1. Label the ALPHA-Carbon, AMINO Group, CARBOXYL Group, and AMINO Side Chain of the following amino acids:- Proline- Glycine- Aspartic acid- Asparagine- Lysine 2. Write the structure of the following polypeptide chainsa.) Asp-Leu-Serb.) Thr-Trpc.) Gln-Ala-Prod.) Ile-Met-Cys-Glu
32) You are working with an molecule with a pl of 6.00 and the pKa's of the ionizable groups on the molecule are 1.3, 4.1 and 7.9. You would like this amino acid to have a net negative charge. Which of the following pH values would result in a net negative charge? A) 1.3 B) 4.1 C) 4.43 D) 6.0 E) 7.9
The isoelectric point of an amino acid is the point where; O a) the amino acid carries no net electrical charge b) the a-carboxylic acid is protonated and the a-amino group is unprotonated c) ) the pKa of the a-carboxylic acid is the same as the pK, of the a-amino group a d) the solubility of the amino acid is maximized e) the a-carboxylic acid and the a-amino group are both half protonated MacBo 80 000 esc F1 F2 F3 F4
2. | Calculate the overall charge (pH 7) on the following three polypeptides and answer the questions below. Assume the following pKa values: N-terminal -NH3®, 7.0; all -COOH groups, 4.0; Arg, 12.5; Cys, 8.4; His, 6.0; Lys, 10.0; Tyr, 10.0. (A) Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg-Arg-Pro-Val-Lys-Val-Tyr-Pro-Asp-Ala -Gly- Glu-Asp-Gln- Ser-Ala-Glu-Ala-Phe-Pro-Leu-Arg-Glu-Phe (B) Ser-Tyr-Ser-Met-Glu–His-Phe-Arg–Trp–Gly-Ala-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu–Val-Tyr-Pro-Asp- Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu-Phe-Cys-Ser-Tyr-Ser-Met-Glu-His-Phe-Asp-Trp-Gly- Asp-Pro-Val-Gly-Pro-Asp-Ala-Gly-Asp-Gln-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu-Val-Tyr-Pro-Asp-Ala | (C) Gly-Ser-Val-Arg-Asp-Pro-Val-Lys-Glu–Val-Tyr-Pro-Asp- Lys-Ala-Gly-Arg-Glu-Ser-Arg-Ala (a) Which of the three peptides would elute first from a gel filtration column? (b) Which of the three peptides would migrate the fastest on SDS-PAGE (c) Which of the three peptides could be…
4. You're working on a structure of a protein and its folding. You think that the interaction between Asp123 and Arg29 is important in determining the structure of the protein. a) What type of amino acid is Asp (acidic, basic, hydrophobic, or polar)? b) What type of amino acid is Arg (acidic, basic, hydrophobic, or polar)? c) What is the strongest interaction that can form between Asp123 and Arg29? You create a mutant Arg29> Lys d) What type of amino acid is Lys (acidic, basic, hydrophobic, or polar)? e) Would you expect this substitution mutation to cause major folding problems? Why or why not? You create mutant Arg29> Glu you discover that this mutant is unable to fold properly, so the protein is nonfunctional. f What type of amino acid is Glu (acidic, basic, hydrophobic, or polar)? g) How does this amino acid substitution cause the protein to fold incorrectly? You find another mutant that also as the same Arg29> Glu mutation. However, this mutant protein įs able to fold normally.…
7. Consider the structures shown. For each structure select all that apply. a) pentose, hexose or heptose b) D or L c) ketose or aldose d) a or ẞ e) pyranose or furanose COOM CHACH Structure A Structure C
Consider a short peptide with the sequence M-C-Q-L-Y-P-E-D-K. List the ionizable groups in this peptide, and the net charge of the whole peptide at pH a) 1.5, b) 7.0, and c)12.0.
Water-soluble proteins such as myoglobin tend to fold such that: A) hydrophobic amino acids R-groups are on the interior of the protein and hydrophilic groups are on the outside OB) all peptides form hydrogen bonds with water. C) hydrophilic amino acid R-groups are on the interior of the protein and hydrophobic groups are on the outside. O D) hydrophilic and hydrophobic amino acid R-groups form hydrogen bonds with each other.
What charged groups are present in lysine at a pH = 7? O A) 1 × NH3¹ B) 1 x COO OC) 1× NH3+ and 1 x COO- Jay OD) 2 × NH3 and 1 × COO- + OE) 1 × NH3+ and 2 × COO- O F) None
For each of the following pairs of amino acids, identify the strongest type of intermolecular forces involved when the side chains interact. Explain in terms of the chemical structures of the amino acid side chains. Use the following list: disulfide bridge, hydrogen bonding, hydrophobic interaction, or salt bridge. a) D and H b) C and C c) L and A d) G and S e) N and T
25) Below are two amino acids: cysteine and histidine. a) Draw the reaction mechanism of a dehydration synthesis reaction between cysteine and histidine below: HS H Product 1 OH ox HN H b) Draw two different products of the dehydration synthesis reaction from part a). Prove that these are two different products by labelling the N and C termini, peptide bond, and R-groups of each product. Product 2 OH

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1. What is the charge of the primary structure of proteins at pH = 2.0, 4.0,6.0 and 11.0 of the following proteins. Draw their structures and predict their net charge. A) Lys- Gly- Ala- Gly B) Lys- Gly-Ala-Glu C) His- Gly- Ala- Glu D) Glu- Gly- Ala -Glu E) Gln-Gly-Ala-Lys