1. What is a calibration curve or calibration plot?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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1. What is a calibration curve or calibration plot?
2. According to the Beer-Lambert law absorbance is proportional
to concentration at each wavelength. In principle, one could
choose any wavelength for quantitative estimations of
concentration. However, we generally select the wavelength of
maximum absorbance for a given sample and use it in our
absorbance measurements. Especially, one should avoid the
steep portions of the spectrum. Why?
3. Which concentration range - a dilute or a concentrated one - is
chosen for accurate determinations of unknown concentrations?
Transcribed Image Text:1. What is a calibration curve or calibration plot? 2. According to the Beer-Lambert law absorbance is proportional to concentration at each wavelength. In principle, one could choose any wavelength for quantitative estimations of concentration. However, we generally select the wavelength of maximum absorbance for a given sample and use it in our absorbance measurements. Especially, one should avoid the steep portions of the spectrum. Why? 3. Which concentration range - a dilute or a concentrated one - is chosen for accurate determinations of unknown concentrations?
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