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1. Turn the specs on to warm up and set the wavelength to 340 nm. Each set of students is assigned a pyruvate (substrate) concentration: Series # 1 Dilution factor Concentration* 0.01 M 1 2 2 3 4 4 5 8 16 5 mM 2.5 mM 1.25 mM 0.625 mM *This is not the final concentration in the cuvette. 2. If you are not assigned S1, dilute your pyruvate accordingly in K Phos pH 7.2 buffer. 3. Create 2 mL of LDH @ dilution factor 10 from the 0.5 mg/mL stock. Write the concentration of LDH @ DF 10 here: μg/mL

1. Turn the specs on to warm up and set the wavelength to 340 nm. Each set of students is assigned a pyruvate (substrate) concentration: Series # 1 Dilution factor Concentration* 0.01 M 1 2 2 3 4 4 5 8 16 5 mM 2.5 mM 1.25 mM 0.625 mM *This is not the final concentration in the cuvette. 2. If you are not assigned S1, dilute your pyruvate accordingly in K Phos pH 7.2 buffer. 3. Create 2 mL of LDH @ dilution factor 10 from the 0.5 mg/mL stock. Write the concentration of LDH @ DF 10 here: μg/mL

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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What is the concentration of LDH at DF 10 

1. Turn the specs on to warm up and set the wavelength to 340 nm. Each set of students is assigned a pyruvate (substrate) concentration: Series # 1 Dilution factor Concentration* 0.01 M 1 2 2 3 4 4 5 8 16 5 mM 2.5 mM 1.25 mM 0.625 mM *This is not the final concentration in the cuvette. 2. If you are not assigned S1, dilute your pyruvate accordingly in K Phos pH 7.2 buffer. 3. Create 2 mL of LDH @ dilution factor 10 from the 0.5 mg/mL stock. Write the concentration of LDH @ DF 10 here: μg/mL
Transcribed Image Text:1. Turn the specs on to warm up and set the wavelength to 340 nm. Each set of students is assigned a pyruvate (substrate) concentration: Series # 1 Dilution factor Concentration* 0.01 M 1 2 2 3 4 4 5 8 16 5 mM 2.5 mM 1.25 mM 0.625 mM *This is not the final concentration in the cuvette. 2. If you are not assigned S1, dilute your pyruvate accordingly in K Phos pH 7.2 buffer. 3. Create 2 mL of LDH @ dilution factor 10 from the 0.5 mg/mL stock. Write the concentration of LDH @ DF 10 here: μg/mL
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