1. Tris(hydroxymethyl)aminomethane abbreviated as Tris or TRIŽMA is frequently used to make buffers in biochemistry laboratories. Tris has a Molecular weight 121.1 g/mol and its solution in water is basic with pH = 10.5. Describe in details how to prepare 1L of 0.5 M Tris buffer solution at pH 7.5. 2. If 2L of 0.05 M Tris-HCI buffer solution pH = 7.5 are required for an experiment on column chromatography. Describe in details how to prepare this buffer solution by diluting the buffer prepared in question # 1. 3. Describe in details how to prepare 10 ml of a 15% (v/v) glycerol solution containing Bovine Serum Albumin (BSA) (3 mg/ml). NH, HO-CH,- CH-OH OH

1. Tris(hydroxymethyl)aminomethane abbreviated as Tris or TRIŽMA is frequently used to make buffers in biochemistry laboratories. Tris has a Molecular weight 121.1 g/mol and its solution in water is basic with pH = 10.5. Describe in details how to prepare 1L of 0.5 M Tris buffer solution at pH 7.5. 2. If 2L of 0.05 M Tris-HCI buffer solution pH = 7.5 are required for an experiment on column chromatography. Describe in details how to prepare this buffer solution by diluting the buffer prepared in question # 1. 3. Describe in details how to prepare 10 ml of a 15% (v/v) glycerol solution containing Bovine Serum Albumin (BSA) (3 mg/ml). NH, HO-CH,- CH-OH OH

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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