1. The microorganism E.coli is grown by the reaction equation below, RQ was measured as 0.85. C6 H12 06 + a NH3 +b02 → a CH1 8 O0.s No.2+ CO2+ SH20 In order to produce 50 g/L of E.coli in a batch reactor of 100,000 liters a. Calculate a,ß,y and d. b. Nutrient media concentration for glucose and ammonium sulfate. c. Calculate Yx/s and Yx/02 d. Calculate total oxygen required
1. The microorganism E.coli is grown by the reaction equation below, RQ was measured as 0.85. C6 H12 06 + a NH3 +b02 → a CH1 8 O0.s No.2+ CO2+ SH20 In order to produce 50 g/L of E.coli in a batch reactor of 100,000 liters a. Calculate a,ß,y and d. b. Nutrient media concentration for glucose and ammonium sulfate. c. Calculate Yx/s and Yx/02 d. Calculate total oxygen required
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
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1. The microorganism E.coli is grown by the reaction equation below, RQ was measured
as 0.85.
C6 H12 06 + a NH3 +b O2→ a CH,8 0.5 No.2+ CO2+ SH20
In order to produce 50 g/L of E.coli in a batch reactor of 100,000 liters
a. Calculate a,B,y and d.
b. Nutrient media concentration for glucose and ammonium sulfate.
c. Calculate Yx/s and Yx/02
d. Calculate total oxygen required
>
2. If in chemostat dilution rate is 0.2 h, culture volume is 350 cm', glucose concentration
in inflow medium is 54 mM and biomass yield per consumed glucose is 0.5 g/g
calculate inflow rate, specific growth rate, doubling time, biomass concentration in
outflow and biomass production rate.
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