1. Provide one reason to support the creation of a national DNA database and one reason to refute its creation. 2. DNA evidence analysis is an imperfect science. Provide two reasons why this might be the case. plssss give at least 2 paragraphs
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1. Provide one reason to support the creation of a national DNA database and one reason to refute its creation.
2. DNA evidence analysis is an imperfect science. Provide two reasons why this might be the case.
plssss give at least 2 paragraphs
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- Examine the gel from a Rape investigation below. If you were the DNA analyst you would conclude that: DNA size markers blood sexual assault evidence samples victim |suspect A suspect B female fracțion male fraction 1 2 3 4 5 6 A. Suspect B is excluded as the source of the evidence, but Suspect A cannot be excluded. B. Both Suspects A and B are excluded as the source of the evidence. C. Suspect A is excluded as the source of the evidence, but Suspect B cannot be excluded. D. Suspect B cannot be excluded as a source of the evidence. The results with Suspect A are inconclusive. E. Neither Suspect A or B can be excluded as a source of the evidence.4. Other reagents used in research labs are listed below. What is the meaning of the acronym and what is its purpose? a. Trypsin b. EDTA C. TEBrenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break? A. Obtaining and handling DNA sample without wearing googles or gloves B. Improper use of human DNA samples C. Violating Patient Confidentiality D. Disposing of bio hazardous material in a regular trash
- 1. Based on the result of spectrophotometry, what can you say about the quantity and purity of your DNA samples? Why did you get such results? 2. Explain the principle involved in the use of 260/280 absorbance ratio for determining the purity of a given DNA sample1. List down at least 5 things that doesn’t have DNA and why? 2. In doing a Laboratory experiment do you think there are tools and components that are more important than the other? Why? Answer question 1 and 2.1. Pharmaceutical industry a. The importance of recombinant DNA technology in pharmaceutical industry b. Potential products produced and processes involved in pharmaceutical industry (at least 5 examples in each field). c. The downside/ disadvantages of recombinant DNA technology in pharmaceutical industry
- 4. state why simplicity and speed are top priorities in selecting DNA isolation methodCollect evidence from 6) the crime scene Probe the membrane with DNA fragments that complement the DNA sequence of the fragments of interest. Compare the fragment profile of the evidence DNA with those of the suspects, detective and victim to see if they match. 2 Isolate DNA from an evidence sample 3 Cut the DNA into fragments using specialized protein "scissors" called restriction enzymes. For every person, the sizes of the cut fragments are unique - except for identical twins. 4 Separate the negatively charged DNA frag- ments in a gel by passing an electric current 8 Re-probe the membrane up to 10 more times to identify different fragments. GEL through it. If the profiles from the evidence DNA and a suspect match multiple times,then it is very likely that the Transfer the DNA fragments from the gel to a sheet evidence DNA came from the suspect. of membrane 1. Describe 3 things that you notice about the process of Gel electrophoresis in the diagram above. 1. 2. 3. 2. How are DNA…A. DNA Sequencing 1. Determine the base sequences of the sample DNA from unknown organisms by examining the bands after gel electrophoresis on photographic films. a. Unknown 1 b. Unknown 2 AGT C I I c. Unknown 3 ||| || | | AGT | || | ||| | | | || I || || ull с I AGT 2. Write down the DNA sequences of each Unknown. I I || d. Unknown 4 || |||||| AGT || ||| | || I || ||| с C ||
- 1. What are some reasons to do PCR? 2. Why do we use a special DNA polymerase from thermophilic bacterium? 3. What are the three temperature ranges used for PCR and what is happening at each step? 4. Why is PCR very susceptible to contamination? all 4 questions please, thanks!DNA evidence first used in US courts.... Between 1980 and 1985 Between 1985 and 1990 Between 1990 and 1995 Between 1995 and 2000 Between 2000 and 20053. Match the following biotech tools with their applications. Some of those tools may have more than one applications. • A. Gene cloning and Recombinant DNA • B. Restriction enzyme • C. Radioactive nucleic acid probe • D. Reverse transcriptase • E. Ti plasmid. • F. Gene therapy • G. PCR • H. Gel electrophoresis • I. single nucleotide polymorphism (SNP) and Restriction fragment length polymorphism (RFLP) • J. Genomic 1. Crime scenes and paternity 2. Making copies of DNA sequence 3. GMO 4. introduce new genes into plant cells 5. alteration of an individual's DNA to treat disease. 6. Cutting DNA at target sequence 7. Detect the presence of specific gene 8. produce a DNA strand from MRNA. 9. separate DNA molecules based on size 10. methods to get the DNA fingerprint of different individual