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1. Osazone test 2. Fehling's test 3. Benedict's test 4. Barfoed's test

1. Osazone test 2. Fehling's test 3. Benedict's test 4. Barfoed's test

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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2. Write the equation involved in all 4 tests above. 1. Osazone test 2. Fehling's test 3. Benedict's test 4. Barfoed's test
Transcribed Image Text:2. Write the equation involved in all 4 tests above. 1. Osazone test 2. Fehling's test 3. Benedict's test 4. Barfoed's test
REACTIONS OF CARBOHYDRATES Reagents:Samples:10% glucose, galactose, fructose, xylose, maltose, sucrose, lactose, starch (5 ml each) ,2 ml Fehling's A, 2 ml Fehling's B, 8 ml Benedict's reagent, 8 ml Barfoed's reagent, 3 g sodium acetate, 2 g phenylhydrazineHCl Materials: 12( 20 ml) test tubes, test tube rack, test tube brush, test tube holder, alcohol lamp, tripod, wire gauze, water bath, (10 ml) graduated cylinder, dropper, stirring rod, beaker(250 ml), spatula, microscope,7 glass slide, 7 cover slip, watch glass, platform balance Procedure: 1. Osazone formation -Mix 3 g sodium acetate and 2 g phenylhydrazineHCL and 14 ml distilled water. Warm with stirring until solution clears. Place 1 ml sugar solution (glucose, fructose, galactose, maltose, xylose, sucrose, lactose) in separate tube. Add 2 ml of the hot solution in each of the sugar solution, stopper with cotton, mix well and heat in a boiling water bath for 30 minutes. Cool at room temperature and examine the crystals under the microscope. Draw and compare the crystals. 2. Fehling's test – mix 2 ml Fehling's A with 2 ml Fehling's B and add 4 ml of water. Boil the solution for 1 minute. If the solution remains clear blue, place 1 ml of the solution in 8 separate test tubes. Add 5 drops of the sugar solution (glucose, fructose, galactose, xylose, maltose, lactose, sucrose, starch)in separate test tubes. Place the tubes in a boiling water bath for another 2 minutes and note the formation and color of precipitate. 3. Benedict's test – place 1 ml of Benedict's reagent in 8 separate test tubes. Add 5 drops of the sugar solution (use all 8 samples) in separate tubes. Place in a boiling water bath for 2 minutes. Cool and observe formation and color of precipitate. 4. Barfoed's test – place 1 ml Barfoed's reagent in 8 separate tubes. Add 5 drops of the sugar solution (use all 8 samples) in separate tubes. Place in a boiling water bath for 5 minutes. Cool and observe formation and color of precipitate.
Transcribed Image Text:REACTIONS OF CARBOHYDRATES Reagents:Samples:10% glucose, galactose, fructose, xylose, maltose, sucrose, lactose, starch (5 ml each) ,2 ml Fehling's A, 2 ml Fehling's B, 8 ml Benedict's reagent, 8 ml Barfoed's reagent, 3 g sodium acetate, 2 g phenylhydrazineHCl Materials: 12( 20 ml) test tubes, test tube rack, test tube brush, test tube holder, alcohol lamp, tripod, wire gauze, water bath, (10 ml) graduated cylinder, dropper, stirring rod, beaker(250 ml), spatula, microscope,7 glass slide, 7 cover slip, watch glass, platform balance Procedure: 1. Osazone formation -Mix 3 g sodium acetate and 2 g phenylhydrazineHCL and 14 ml distilled water. Warm with stirring until solution clears. Place 1 ml sugar solution (glucose, fructose, galactose, maltose, xylose, sucrose, lactose) in separate tube. Add 2 ml of the hot solution in each of the sugar solution, stopper with cotton, mix well and heat in a boiling water bath for 30 minutes. Cool at room temperature and examine the crystals under the microscope. Draw and compare the crystals. 2. Fehling's test – mix 2 ml Fehling's A with 2 ml Fehling's B and add 4 ml of water. Boil the solution for 1 minute. If the solution remains clear blue, place 1 ml of the solution in 8 separate test tubes. Add 5 drops of the sugar solution (glucose, fructose, galactose, xylose, maltose, lactose, sucrose, starch)in separate test tubes. Place the tubes in a boiling water bath for another 2 minutes and note the formation and color of precipitate. 3. Benedict's test – place 1 ml of Benedict's reagent in 8 separate test tubes. Add 5 drops of the sugar solution (use all 8 samples) in separate tubes. Place in a boiling water bath for 2 minutes. Cool and observe formation and color of precipitate. 4. Barfoed's test – place 1 ml Barfoed's reagent in 8 separate tubes. Add 5 drops of the sugar solution (use all 8 samples) in separate tubes. Place in a boiling water bath for 5 minutes. Cool and observe formation and color of precipitate.
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