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1. Obtain five unknown carbohydrates samples from your laboratory instructor. 2. Place approximately 2 ml of each sample in separate test tubes. Use fresh sample for each qualitative test. a. For Barfoed's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the change. b. For Seliwanoff's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. c. For Benedict's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. d. For Lugol's test, add 1 ml of the test regent and mix well. Observe and record the color change. 3. The samples used are the following: fructose, lactose, wheat flour, table sugar, and ribose. 4. Match the samples with the obtained reaction profile in Table 1. State the reason(s) why you match each sample with a particular reaction profile.

1. Obtain five unknown carbohydrates samples from your laboratory instructor. 2. Place approximately 2 ml of each sample in separate test tubes. Use fresh sample for each qualitative test. a. For Barfoed's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the change. b. For Seliwanoff's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. c. For Benedict's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. d. For Lugol's test, add 1 ml of the test regent and mix well. Observe and record the color change. 3. The samples used are the following: fructose, lactose, wheat flour, table sugar, and ribose. 4. Match the samples with the obtained reaction profile in Table 1. State the reason(s) why you match each sample with a particular reaction profile.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Identify the unknown carbohydrate sample and give it's rationale

1. Obtain five unknown carbohydrates samples from your laboratory instructor. 2. Place approximately 2 ml of each sample in separate test tubes. Use fresh sample for each qualitative test. a. For Barfoed's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the change. b. For Seliwanoff's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. c. For Benedict's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. d. For Lugol's test, add 1 ml of the test regent and mix well. Observe and record the color change. 3. The samples used are the following: fructose, lactose, wheat flour, table sugar, and ribose. 4. Match the samples with the obtained reaction profile in Table 1. State the reason(s) why you match each sample with a particular reaction profile.
Transcribed Image Text:1. Obtain five unknown carbohydrates samples from your laboratory instructor. 2. Place approximately 2 ml of each sample in separate test tubes. Use fresh sample for each qualitative test. a. For Barfoed's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the change. b. For Seliwanoff's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. c. For Benedict's test, add 1 ml of the test reagent and heat the samples in a water bath for 5 minutes. Observe and record the color change. d. For Lugol's test, add 1 ml of the test regent and mix well. Observe and record the color change. 3. The samples used are the following: fructose, lactose, wheat flour, table sugar, and ribose. 4. Match the samples with the obtained reaction profile in Table 1. State the reason(s) why you match each sample with a particular reaction profile.
Sample No. 1 2 3 4 LO 5 Barfoed's Test Light blue/aqua- colored solution Green-colored solution forming a brick-red precipitate at the bottom Light blue/aqua- colored solution Blue-green colored solution forming a brick- red precipitate at the bottom Light blue/aqua- colored solution Seliwanoff's Test Pale yellow- colored solution Cherry red- colored solution Pale yellow- colored solution Pale yellow- colored solution Pale yellow- colored solution Benedict's Test Rust-colored precipitates Rust-colored precipitates Aqua blue-colored solution Rust-colored precipitates Aqua blue-colored solution Lugol's Test Brown-colored solution Brown-colored solution Blue-black- colored solution Brown-colored solution Brown-colored solution
Transcribed Image Text:Sample No. 1 2 3 4 LO 5 Barfoed's Test Light blue/aqua- colored solution Green-colored solution forming a brick-red precipitate at the bottom Light blue/aqua- colored solution Blue-green colored solution forming a brick- red precipitate at the bottom Light blue/aqua- colored solution Seliwanoff's Test Pale yellow- colored solution Cherry red- colored solution Pale yellow- colored solution Pale yellow- colored solution Pale yellow- colored solution Benedict's Test Rust-colored precipitates Rust-colored precipitates Aqua blue-colored solution Rust-colored precipitates Aqua blue-colored solution Lugol's Test Brown-colored solution Brown-colored solution Blue-black- colored solution Brown-colored solution Brown-colored solution
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