1. For this mixture of polyaromatic hydrocarbons (PAH's), which LC column stationary phase would be expected to give the best separation? C18 Phenyl Cyano www m

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
icon
Related questions
icon
Concept explainers
Question
Give answer all questions with explanation and answer no. Please
1. For this mixture of polyaromatic hydrocarbons (PAH's), which LC column stationary phase would be
expected to give the best separation?
C18
Phenyl
Cyano
Explain the basis of your choice.
2. Using a C18 HPLC column and a mobile phase with 10% acetonitrile, the retention times of a mixture
are 2.35 minutes. 3.78 minutes and 5.72 minutes. If the acetonitrile content is increased to 15%, would
you expect these retention times to increase or decrease? Explain your answer.
3. Size exclusion chromatography (SEC) relies on separation associated with how far an analyte
penetrates into the pores in the stationary phase. It is used to separate species with molecular weights
much greater than 1000 Daltons. How does the retention time vary with molecular weight in an SEC
separation of molecules with similar chemical structures?
Transcribed Image Text:1. For this mixture of polyaromatic hydrocarbons (PAH's), which LC column stationary phase would be expected to give the best separation? C18 Phenyl Cyano Explain the basis of your choice. 2. Using a C18 HPLC column and a mobile phase with 10% acetonitrile, the retention times of a mixture are 2.35 minutes. 3.78 minutes and 5.72 minutes. If the acetonitrile content is increased to 15%, would you expect these retention times to increase or decrease? Explain your answer. 3. Size exclusion chromatography (SEC) relies on separation associated with how far an analyte penetrates into the pores in the stationary phase. It is used to separate species with molecular weights much greater than 1000 Daltons. How does the retention time vary with molecular weight in an SEC separation of molecules with similar chemical structures?
Expert Solution
steps

Step by step

Solved in 3 steps

Blurred answer
Knowledge Booster
Introduction to Organic Chemistry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY