1. A protein chromatography column that processes 15 kg of protein in 60 h, is cycled every 6 h, can be loaded with 10 grams of protein per liter of column volume, has a maximum flow rate of 50 liters/ min at a pressure of 4 bar, and is packed with an incompressible resin having a particle diameter of 90 μum and void fraction of 0.35. The viscosity can be assumed to be that of water. i. What are the dimensions required for the column? ii. If the flow rate is 1 column volume per minute and the pressure rating is still 4 bar, what column dimensions are required? Is the resolution equivalent to that for Part (i)? 111. If the pressure rating for the column were raised to 12 bar, should the column dimensions change? Is so, what should the dimensions be? Do they make sense? iv. What is the best way to increase the productivity of the column packing?
1. A protein chromatography column that processes 15 kg of protein in 60 h, is cycled every 6 h, can be loaded with 10 grams of protein per liter of column volume, has a maximum flow rate of 50 liters/ min at a pressure of 4 bar, and is packed with an incompressible resin having a particle diameter of 90 μum and void fraction of 0.35. The viscosity can be assumed to be that of water. i. What are the dimensions required for the column? ii. If the flow rate is 1 column volume per minute and the pressure rating is still 4 bar, what column dimensions are required? Is the resolution equivalent to that for Part (i)? 111. If the pressure rating for the column were raised to 12 bar, should the column dimensions change? Is so, what should the dimensions be? Do they make sense? iv. What is the best way to increase the productivity of the column packing?
Essentials Of Investments
11th Edition
ISBN:9781260013924
Author:Bodie, Zvi, Kane, Alex, MARCUS, Alan J.
Publisher:Bodie, Zvi, Kane, Alex, MARCUS, Alan J.
Chapter1: Investments: Background And Issues
Section: Chapter Questions
Problem 1PS
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Transcribed Image Text:1. A protein chromatography column that processes 15 kg of protein in 60 h, is cycled
every 6 h, can be loaded with 10 grams of protein per liter of column volume, has a
maximum flow rate of 50 liters/ min at a pressure of 4 bar, and is packed with an
incompressible resin having a particle diameter of 90 μum and void fraction of 0.35. The
viscosity can be assumed to be that of water.
i.
What are the dimensions required for the column?
ii.
If the flow rate is 1 column volume per minute and the pressure rating is still 4
bar, what column dimensions are required? Is the resolution equivalent to that
for Part (i)?
111.
If the pressure rating for the column were raised to 12 bar, should the column
dimensions change? Is so, what should the dimensions be? Do they make sense?
iv.
What is the best way to increase the productivity of the column packing?
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