Aiman is trying to isolate intracellular protein of a recombinant microorganism growing in hisresearch laboratory. The protein is said to be used in medical sector and cannot withstand temperature for more than 40\deg C and has a size of 100kD. As a chemical engineer friend to Kamal,could you advise him on:a) His first option is to recover the intracellular protein using osmotic pressure. The pressure dropbetween with outside and inside concentrations are 0.01M and 0.2M respectively, what is thepressure drop value of if the salts are disassociate completely.(5 marks) b) What can be said of this pressure drop value and why?(5 marks)c) His second option is to use a pressure valve homogenizer. What is the cell breakage at 0.9? Assuming the value 1.77 for of k = 5.91 x 10* MPa and a the range pressure of50MPa. Please show all work.

Essentials Of Investments
11th Edition
ISBN:9781260013924
Author:Bodie, Zvi, Kane, Alex, MARCUS, Alan J.
Publisher:Bodie, Zvi, Kane, Alex, MARCUS, Alan J.
Chapter1: Investments: Background And Issues
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Aiman is trying to isolate intracellular
protein of a recombinant microorganism
growing in hisresearch laboratory. The
protein is said to be used in medical
sector and cannot withstand temperature
for more than 40\deg C and has a size of
100kD. As a chemical engineer friend to
Kamal,could you advise him on:a) His first
option is to recover the intracellular
protein using osmotic pressure. The
pressure dropbetween with outside and
inside concentrations are 0.01M and 0.2M
respectively, what is thepressure drop
value of if the salts are disassociate
completely.(5 marks) b) What can be said
of this pressure drop value and why?(5
marks)c) His second option is to use a
pressure valve homogenizer. What is the
cell breakage at 0.9? Assuming the value
1.77 for
of k = 5.91 x 10* MPa and a
the range pressure of50MPa. Please show
all work.
Transcribed Image Text:Aiman is trying to isolate intracellular protein of a recombinant microorganism growing in hisresearch laboratory. The protein is said to be used in medical sector and cannot withstand temperature for more than 40\deg C and has a size of 100kD. As a chemical engineer friend to Kamal,could you advise him on:a) His first option is to recover the intracellular protein using osmotic pressure. The pressure dropbetween with outside and inside concentrations are 0.01M and 0.2M respectively, what is thepressure drop value of if the salts are disassociate completely.(5 marks) b) What can be said of this pressure drop value and why?(5 marks)c) His second option is to use a pressure valve homogenizer. What is the cell breakage at 0.9? Assuming the value 1.77 for of k = 5.91 x 10* MPa and a the range pressure of50MPa. Please show all work.
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