1. A microbiologist sets up a serial dilution to analyze bacteria in raw milk. Tube 1-0.1 mL milk diluted in 9.9 mL sterile water Tube 2-0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water Tube 3– 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water What is the dilution of raw milk in Tube 3?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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1. A microbiologist sets up a serial dilution to analyze bacteria in raw milk.
Tube 1-0.1 mL milk diluted in 9.9 mL sterile water
Tube 2-0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water
Tube 3- 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water
What is the dilution of raw milk in Tube 3?
2. A researcher analyzes unpasteurized apple juice suspecting E. coli
contamination of the juice. He/she plates out 0.1 mL of a 102 dilution of juice on
each plate and grows up the plates. He/she then counts colonies on each plate.
Plate 1- 242 colonies
Plate 2 - 237 colonies
Plate 3- 249 colonies
|
(a) Calculate the CFU/mL for this juice. Show formula used and include units.
(b) If acceptable levels of E. coli in juice were 0.02 CFU/ml juice, would this juice
be acceptable for human consumption?
(1)
Why or why not?
d States)
t9 AON
étv
24
MacBook Pro
Transcribed Image Text:1. A microbiologist sets up a serial dilution to analyze bacteria in raw milk. Tube 1-0.1 mL milk diluted in 9.9 mL sterile water Tube 2-0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water Tube 3- 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water What is the dilution of raw milk in Tube 3? 2. A researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice. He/she plates out 0.1 mL of a 102 dilution of juice on each plate and grows up the plates. He/she then counts colonies on each plate. Plate 1- 242 colonies Plate 2 - 237 colonies Plate 3- 249 colonies | (a) Calculate the CFU/mL for this juice. Show formula used and include units. (b) If acceptable levels of E. coli in juice were 0.02 CFU/ml juice, would this juice be acceptable for human consumption? (1) Why or why not? d States) t9 AON étv 24 MacBook Pro
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