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1-3 Aseptic Technique 1. What's the purpose of using aseptic technique (multiple reasons)? 2. What part of the Bunsen burner flame is best for inoculating the loop the outer cone or inner cone? Why? 3. When opening a plate, you should use the as a shield to minimize 4. List the steps in order that are used to transfer bacteria from one test tube to another.

1-3 Aseptic Technique 1. What's the purpose of using aseptic technique (multiple reasons)? 2. What part of the Bunsen burner flame is best for inoculating the loop the outer cone or inner cone? Why? 3. When opening a plate, you should use the as a shield to minimize 4. List the steps in order that are used to transfer bacteria from one test tube to another.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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bio 2 from Lab Manual 4 TH edition Leboffe and Pierce 

## Aseptic Technique 1. **What's the purpose of using aseptic technique (multiple reasons)?** 2. **What part of the Bunsen burner flame is best for inoculating the loop – the outer cone or inner cone? Why?** 3. **When opening a plate, you should use the _______________ as a shield to minimize ___________.** 4. **List the steps in order that are used to transfer bacteria from one test tube to another.** This excerpt outlines several fundamental questions about aseptic techniques, commonly employed in laboratories to prevent contamination of samples and ensure the accuracy of experimental results. Students should focus on understanding each of these aspects to effectively practice aseptic methods in microbiological work. ### Explanation of Concepts: 1. **Purpose of Aseptic Technique:** - Prevent contamination of cultures and media from microbes in the environment. - Protect laboratory personnel from exposure to potentially harmful organisms. - Prevent contamination of the environment with potentially harmful organisms. - Ensure no changes in experiment results due to unintended introduction of external organisms. 2. **Bunsen Burner Flame for Inoculating Loop:** - The best part of the flame for inoculating the loop is the inner cone. The inner cone of the Bunsen burner flame is the hottest part, which ensures that any contaminants on the loop are rapidly incinerated, thus sterilizing the loop effectively. 3. **Using a Shield When Opening a Plate:** - When opening a plate, you should use the lid as a shield to minimize airborne contamination. 4. **Steps to Transfer Bacteria:** - **Flame sterilize** the inoculating loop. - **Flame sterilize** the mouth of the culture tube. - **Dip the sterilized loop** into the culture. - **Flame sterilize** the mouth of the receiving tube. - **Transfer the bacteria** by dipping the loop into the receiving tube. - **Flame sterilize** the mouth of the receiving tube and close it. - **Flame sterilize** the inoculating loop before setting it aside. Mastering these steps and concepts will enhance lab accuracy and safety, crucial for conducting reliable and responsible scientific research.
Transcribed Image Text:## Aseptic Technique 1. **What's the purpose of using aseptic technique (multiple reasons)?** 2. **What part of the Bunsen burner flame is best for inoculating the loop – the outer cone or inner cone? Why?** 3. **When opening a plate, you should use the _______________ as a shield to minimize ___________.** 4. **List the steps in order that are used to transfer bacteria from one test tube to another.** This excerpt outlines several fundamental questions about aseptic techniques, commonly employed in laboratories to prevent contamination of samples and ensure the accuracy of experimental results. Students should focus on understanding each of these aspects to effectively practice aseptic methods in microbiological work. ### Explanation of Concepts: 1. **Purpose of Aseptic Technique:** - Prevent contamination of cultures and media from microbes in the environment. - Protect laboratory personnel from exposure to potentially harmful organisms. - Prevent contamination of the environment with potentially harmful organisms. - Ensure no changes in experiment results due to unintended introduction of external organisms. 2. **Bunsen Burner Flame for Inoculating Loop:** - The best part of the flame for inoculating the loop is the inner cone. The inner cone of the Bunsen burner flame is the hottest part, which ensures that any contaminants on the loop are rapidly incinerated, thus sterilizing the loop effectively. 3. **Using a Shield When Opening a Plate:** - When opening a plate, you should use the lid as a shield to minimize airborne contamination. 4. **Steps to Transfer Bacteria:** - **Flame sterilize** the inoculating loop. - **Flame sterilize** the mouth of the culture tube. - **Dip the sterilized loop** into the culture. - **Flame sterilize** the mouth of the receiving tube. - **Transfer the bacteria** by dipping the loop into the receiving tube. - **Flame sterilize** the mouth of the receiving tube and close it. - **Flame sterilize** the inoculating loop before setting it aside. Mastering these steps and concepts will enhance lab accuracy and safety, crucial for conducting reliable and responsible scientific research.
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