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1-11 Exactly 5.00-mL aliquots of a solution containing phenobarbital were measured into 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of phenobarbital containing 2.000 ug/mL of phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50, and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, which gave values of 3.26, 4.80, 6.41, 8.02, and 9.56, respectively. (a) Plot the data. *(b) Using the plot from (a), calculate the concentration of phenobarbital in the unknown.

1-11 Exactly 5.00-mL aliquots of a solution containing phenobarbital were measured into 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of phenobarbital containing 2.000 ug/mL of phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50, and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, which gave values of 3.26, 4.80, 6.41, 8.02, and 9.56, respectively. (a) Plot the data. *(b) Using the plot from (a), calculate the concentration of phenobarbital in the unknown.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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1-11 Exactly 5.00-mL aliquots of a solution containing phenobarbital were measured into 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of phenobarbital containing 2.000 µg/mL of phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50, and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, which gave values of 3.26, 4.80, 6.41, 8.02, and 9.56, respectively. (a) Plot the data. *(b) Using the plot from (a), calculate the concentration of phenobarbital in the unknown.
Transcribed Image Text:1-11 Exactly 5.00-mL aliquots of a solution containing phenobarbital were measured into 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of phenobarbital containing 2.000 µg/mL of phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50, and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, which gave values of 3.26, 4.80, 6.41, 8.02, and 9.56, respectively. (a) Plot the data. *(b) Using the plot from (a), calculate the concentration of phenobarbital in the unknown.
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