.columns with one million theoretical plates operating by slip flow. The figure shows the gradient separation of two forms of fluorescently labeled bovine serum albumin separated on a)50 x 2.1 mm UHPLC column of 1.7 µm C4 silica particles and b)10 mm x 75µm colloidal crystal column of 470 nm C4 silica nanoparticles. What are the main differences in the chromatogram? What is the reason for the difference? Why does the colloidal crystal column have such a high number of theoretical plates (N)? How is the problem of high pressures with very small particles overcome?
.columns with one million theoretical plates operating by slip flow. The figure shows the gradient separation of two forms of fluorescently labeled bovine serum albumin separated on a)50 x 2.1 mm UHPLC column of 1.7 µm C4 silica particles and b)10 mm x 75µm colloidal crystal column of 470 nm C4 silica nanoparticles. What are the main differences in the chromatogram? What is the reason for the difference? Why does the colloidal crystal column have such a high number of theoretical plates (N)? How is the problem of high pressures with very small particles overcome?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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4.columns with one million theoretical plates operating by slip flow.
The figure shows the gradient separation of two forms of fluorescently labeled bovine serum albumin separated on
a)50 x 2.1 mm UHPLC column of 1.7 µm C4 silica particles and
b)10 mm x 75µm colloidal crystal column of 470 nm C4 silica nanoparticles.
- What are the main differences in the chromatogram? What is the reason for the difference?
- Why does the colloidal crystal column have such a high number of theoretical plates (N)?
- How is the problem of high pressures with very small particles overcome?
Transcribed Image Text:10
11
12
13
2.2
2.3
2.4
2.5
2.6
2.7
(d
Time (min)
Time (min)
(a)
(b)
Detector response
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