Pulmonary Function Data Worksheet for Respiratory Physiology

Respiratory Physiology Data Worksheet Page 1 of 3 Complete 1 worksheet per subject. Variables with a red box around them will be recorded on a Google sheet. Submit your worksheets with your post-lab assignment. Pulmonary Function Part I. Subject profile. Age: ___________ Height: __________ cm Sex: Male / Female A) Vital Capacity (V.C.) i) Predicted: Use the equation below to calculate the Predicted Vital Capacity: ________________ L Equations for Predicted Vital Capacity (from Kory, Hamilton, Callahan 1960) Male: Predicted V.C. = 0.052H – 0.022A – 3.60 Female: Predicted V.C. = 0.041H – 0.018A – 2.69 H = Height in centimeters A = Age ii) Observed: The P-P result is the Observed Vital Capacity:________________ iii) Observed vs Predicted. What is the Subject’s observed Vital Capacity as a percentage of the predicted Vital Capacity? Observed/Predicted V.C. = __________________ x 100 = ________________% Note : Vital capacities are dependent on other factors besides age and height. Therefore, 80% of predicted values are still considered ! normal.” B) Volume and Capacity Measurements Parameter Measurement Result Calculations Tidal Volume (a + b + c + d) / 4 = ____________ Inspiratory Reserve Volume (IRV) __________________ Expiratory Reserve Volume (ERV) __________________ Inspiratory Capacity (IC) TV + IRV = ______________ Expiratory Capacity (EC) TV + ERV = ______________ Residual Volume Assume 1 L Functional Residual Capacity ERV + RV = ______________ Total Lung Capacity IRV + TV + ERV + RV = ________________ 5.45 5.13 0.941 94.1 0.991 1.09 0.924 0.825 0.958 2.67 L 1.65 3.63 2.61 2.65 6.28 21 183

Respiratory Physiology Data Worksheet Page 2 of 3 Pulmonary Function Part II. Subject profile. Age: _____________ Height: ____________cm Sex: Male / Female A) Forced Vital Capacity = ____________________ L B) Forced Expired Volumes (FEV 1.0 , FEV 2.0 , FEV 3.0 ) FEV x Time Interval (sec) Forced Expired Volume (L) Forced Vital Capacity (L)(from Part A) FEV/FVC (calculate) (FEV/FVC) x 100 = % (calculate) Normal Adult Range FEV 1.0 0 – 1 66 – 83% FEV 2.0 0 – 2 75 – 94% FEV 3.0 0 – 3 78 – 97% C) MVV Measurements 1) Count the number of cycles in a 12-second interval: ____________________ 2) Calculate the number of respiratory cycles per minute (RR): RR = # cycles in a 12-second interval x 5 = _____________________ 3) Measure the volume of each cycle. Complete the table with a measurement for each cycle the subject completed. Do not record incomplete cycles. (The table may have more cycles than you need). Cycle number Volume measurement (L) Cycle number Volume measurement (L) 1 9 2 10 3 11 4 12 5 13 6 14 7 15 8 16 35 7 2.92 3.00 2.90 3.08 3.43 3.39 3.81 21 183 4.77 3.86 4.59 4.76 4.77 4.77 4.77 0.809 0.962 0.998 80.9 % 96.2 % 99.8 %

Respiratory Physiology Data Worksheet Page 3 of 3 4) Calculate the average volume per cycle (AVPC). Add the volumes of all counted cycles from the table. Sum = _________________ L AVPC = ___________ / _____________ = ____________ L Sum # of cycles 5) Calculate the MVV est Multiple the AVPC by the number of respiratory cycles per minute (RR) MVV est = _________ x ________ = _______________ L/min AVPC RR You will combine your data with other lab sections from the same day. Enter the following data into the Google spreadsheet for your lab day for each subject (report all volumes in L): From Part I: Age, height, sex, Observed/Predicted VC%, Total Lung Capacity From Part II: Age, height, sex, FEV 1.0 , FVC, FEV 1.0 /FVC (%), MVVest DO NOT include names, group numbers or other identifiers. Monday & Tuesday labs: http://bit.ly/RespPhysTues Wednesday labs: http://bit.ly/RespPhysWed Thursday labs: http://bit.ly/RespPhysThurs Friday labs: http://bit.ly/RespPhysFri 7 22.5 3.22 35 3.22 113 22.5

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Respiratory Physiology Data Worksheet Page 1 of 3 Complete 1 worksheet per subject. Variables with a red box around them will be recorded on a Google sheet. Submit your worksheets with your post-lab assignment. Pulmonary Function Part I. Subject profile. Age: ___________ Height: __________ cm Sex: Male / Female A) Vital Capacity (V.C.) i) Predicted: Use the equation below to calculate the Predicted Vital Capacity: ________________ L Equations for Predicted Vital Capacity (from Kory, Hamilton, Callahan 1960) Male: Predicted V.C. = 0.052H – 0.022A – 3.60 Female: Predicted V.C. = 0.041H – 0.018A – 2.69 H = Height in centimeters A = Age ii) Observed: The P-P result is the Observed Vital Capacity:________________ iii) Observed vs Predicted. What is the Subject’s observed Vital Capacity as a percentage of the predicted Vital Capacity? Observed/Predicted V.C. = __________________ x 100 = ________________% Note : Vital capacities are dependent on other factors besides age and height. Therefore, 80% of predicted values are still considered ! normal.” B) Volume and Capacity Measurements Parameter Measurement Result Calculations Tidal Volume (a + b + c + d) / 4 = ____________ Inspiratory Reserve Volume (IRV) __________________ Expiratory Reserve Volume (ERV) __________________ Inspiratory Capacity (IC) TV + IRV = ______________ Expiratory Capacity (EC) TV + ERV = ______________ Residual Volume Assume 1 L Functional Residual Capacity ERV + RV = ______________ Total Lung Capacity IRV + TV + ERV + RV = ________________ 4.90 21 183 5.45 0.899 89.9 1.12 3.11 4.90 0.754 1.00 0.821 0.924 4.03 5.82 5.90 9.93

Respiratory Physiology Data Worksheet Page 2 of 3 Pulmonary Function Part II. Subject profile. Age: _____________ Height: ____________cm Sex: Male / Female A) Forced Vital Capacity = ____________________ L B) Forced Expired Volumes (FEV 1.0 , FEV 2.0 , FEV 3.0 ) FEV x Time Interval (sec) Forced Expired Volume (L) Forced Vital Capacity (L)(from Part A) FEV/FVC (calculate) (FEV/FVC) x 100 = % (calculate) Normal Adult Range FEV 1.0 0 – 1 66 – 83% FEV 2.0 0 – 2 75 – 94% FEV 3.0 0 – 3 78 – 97% C) MVV Measurements 1) Count the number of cycles in a 12-second interval: ____________________ 2) Calculate the number of respiratory cycles per minute (RR): RR = # cycles in a 12-second interval x 5 = _____________________ 3) Measure the volume of each cycle. Complete the table with a measurement for each cycle the subject completed. Do not record incomplete cycles. (The table may have more cycles than you need). Cycle number Volume measurement (L) Cycle number Volume measurement (L) 1 9 2 10 3 11 4 12 5 13 6 14 7 15 8 16 4.93 4.93 4.93 4.55 4.93 0.809 0.962 0.998 92.3 % 100 % 100 % 4.93 4.93 11 55 1.96 2.37 2.02 1.65 1.70 1.92 1.69 1.86 1.95 2.07 2.09 21 183

Respiratory Physiology Data Worksheet Page 3 of 3 4) Calculate the average volume per cycle (AVPC). Add the volumes of all counted cycles from the table. Sum = _________________ L AVPC = ___________ / _____________ = ____________ L Sum # of cycles 5) Calculate the MVV est Multiple the AVPC by the number of respiratory cycles per minute (RR) MVV est = _________ x ________ = _______________ L/min AVPC RR You will combine your data with other lab sections from the same day. Enter the following data into the Google spreadsheet for your lab day for each subject (report all volumes in L): From Part I: Age, height, sex, Observed/Predicted VC%, Total Lung Capacity From Part II: Age, height, sex, FEV 1.0 , FVC, FEV 1.0 /FVC (%), MVVest DO NOT include names, group numbers or other identifiers. Monday & Tuesday labs: http://bit.ly/RespPhysTues Wednesday labs: http://bit.ly/RespPhysWed Thursday labs: http://bit.ly/RespPhysThurs Friday labs: http://bit.ly/RespPhysFri 21.27 21.27 11 1.93 106.2 1.93 55

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2. Graph #1: Average observed/predicted VC (%) males v. females Legend: A bar graph depicting average observed/predicted VC (%) values for males versus females was plotted. The p-value can be obtained using an unpaired T-Test which results in a p-value of 0.104. Since p>0.05, the data is not statistically significant. 3. Graph #2: Scatterplot of Total Lung Capacity (L) and Height (cm) Legend: A scatterplot is depicted of total lung capacity versus height. The p-value is 0.000131 and since p<0.05, the results are statistically significant. The linear regression line had the equation y = 0.0983x - 11.416 with an R 2 value of 0.2801. 0 10 20 30 40 50 60 70 80 90 100 M F Average Observed/Predicted VC (%) Sex (M/F) y = 0.0983x - 11.416 R ² = 0.2801 0 2 4 6 8 10 12 150 160 170 180 190 200 Total Lung Capacity (L) Height (cm) Total Lung Capacity (L) Linear (Total Lung Capacity (L))

4. Graph #3: Scatterplot of Relationship between FEV1.0/FVC (%) and Height (cm) Legend : A scatterplot is plotted using FEV1.0/FVC (%) versus height (cm). It has a p- value of 0.331 and since p>0.05, the results are not statistically significant. The linear regression line has the equation y = -0.3023x +110.4 with an R 2 value of 0.0215. 5. Graph #4: Bar Graph of average MVV est (L/min) of Males v. Females Legend: A bar graph is plotted to depict the average MVV est (L/min) of males versus females. A p-value of 4.07e-8 was the result after performing an unpaired T-test. Since p<0.05, the results were statistically significant. 6a. Obstructive lung diseases are more likely to have low values of observed/predicted VC %. y = -0.3023x + 110.4 R² = 0.0215 0 10 20 30 40 50 60 70 80 90 100 150 155 160 165 170 175 180 185 190 195 FEV1.0/FVC (%) Height (cm) FEV1.0/FVC (%) Linear (FEV1.0/FVC (%)) 0 20 40 60 80 100 120 140 160 M F Average MVV est (L/min) Sex (M/F)

6b. When someone hyperventilates, they could pass out briefly because they expel more CO2 from their body which leads to CO2 in blood to drop below the normal value.

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Week 1 Respiratory Physiology Assignment

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