BIOS242 Week 1 Handwashing Lab

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School

Chamberlain University College of Nursing *

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Course

242

Subject

Mechanical Engineering

Date

Dec 6, 2023

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docx

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3

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BIOS242 Lab 1 Name: Lab 1: Importance of Handwashing Learning Objectives: Explain effectiveness of hand washing GI infections occur due to contaminated food and water. Clean drinking water, washed produce, appropriate storage of food and simple personal hygiene habits such as hand washing help tremendously in reducing the incident of GI infections. In this lab, we will test the effectiveness of some of these practices. Note: Use personal protective equipment (PPE) at all times. Follow instructions at all times. After overnight growth, seal the plates with parafilm. Do not open the plates. Exercise 1: Personal Hygiene- Importance of Hand washing Materials: Nutrient agar, ruler, marker, hand soap, paper towel Method: 1. Obtain a nutrient agar plate. 2. Use a marker and a ruler to divide the back of the agar plate in half. Mark each half as “Before Washing” and “After Washing”. 3. Slightly open the “Before Washing” section of the plate and without washing your hands, gently touch your index finger on the agar plate for approximately 3 seconds. a. Do not press too hard to disturb the agar b. Gently touch your finger to agar; there is no need to press. 4. Wash your hands thoroughly with soap and water. 5. Gently blot dry your hands using paper towel. 6. Slightly open the “After Washing” section of the plate and gently touch your index finger on the agar plate for approximately 3 seconds. 7. Keep the plates in incubator at 37 C for 24 hours. 8. During the next lab meeting, count the colonies grown in each of the sections of your plate. If it is more than 100 colonies, write TNTC (Too numerous to count) in the report. 9. Write your observations in Lab report. 10. Once observation is completed, immediately put the plates in appropriate waste container for autoclaving. 1
BIOS242 Lab 1 Name: Lab Report: Purpose: Describe the purpose of this lab. Hand washing is an essential component of laboratory safety. The purpose of this lab was to detect the microorganisms present in our hands before and after hand washing. Exercise 1: Personal Hygiene- Importance of Hand washing Draw your observations of bacterial growth: Complete the following table. Before Hand washing After Hand Washing Number of colonies Describe your observations in the space below. Upon viewing the plate after incubation, it is observed that the number of colonies were more and too numerous too count before washing compared to after. This goes to show that hand washing is effective and vital top prevent the spread of germs. Questions: 1. You are a nurse educator training novice nurse trainees the importance of hand washing in clinical setting. Why is it important to have proper hand washing technique? 2
BIOS242 Lab 1 Name: Washing your hands properly with soap and water can help prevent the spread of the germs (like bacteria and viruses) that cause these diseases. By washing our hands, we are reducing the risk of spreading some forms of gastrointestinal and respiratory infections that can cause serious complications, especially for young children, the elderly, or those with a weakened immune system. Hence, why is is advisable to healthcare professionals as well as the public to wash their hands frequently to reduce the spread of germs. Grading Rubric: Activity Deliverable Points Experimental Set up Set up the experiment as directed in the lab or by instructor 4 Observation Observe the results and record them in lab report 4 Lab Report and Questions Complete lab report and answer questions 7 All Lab Deliverables Complete ALL lab work and lab report 15 3
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