Week 2 Virtual Lab

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Chamberlain University College of Nursing *

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256

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Mechanical Engineering

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Feb 20, 2024

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docx

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BIOS256 OL, Week 2 Lab Name: OL Lab 2: Intestinal Glucose Transport: Study a mouse intestine model to diagnose an infant. Learning Objectives:  Understand the epithelial model for how glucose is transported across the mammalian small intestine  Perform a glucose assay method  Use an animal model to study the transport of materials across the intestine  Describe the effect of sodium-potassium ATPase blocker on glucose transport by the small intestine  Explain the effect of manipulations of mucosal concentrations of glucose and sodium on glucose intestinal transport  Interpret physiological data and apply to clinical cases In this simulation, you will learn how glucose absorption occurs in the intestine. You will help treat an infant who has trouble gaining weight. She is experiencing diarrhea, with elevated blood sodium concentration and glucose in her feces and urine. Dr. Shaw, your supervisor, suspects this could be related to the poor glucose absorption. You will use a piece of mouse intestine as your model to study intestinal glucose transport and see how your findings can be used to diagnose human diseases. Study glucose transport by measuring its concentration: After clearing out the impurities from the everted intestinal piece, you will expose both the inner (mucosal) and outer (serosal) sides to glucose solutions of different concentrations, a sodium- potassium ATPase blocker, and a sodium-free saline solution. After these treatments, you will measure the glucose levels on both sides, and analyze your data to understand the active glucose transport through the intestinal epithelium. Also, a step- by-step interactive summary diagram will help you visualize what happens to all the molecules and transporters involved. Will your findings be able to help make the infant well again? Part 1: Complete Labster- Intestinal Glucose Transport: Study a mouse intestine model to diagnose an infant Part 2: Report and Reflection Purpose: Describe in your own words and in complete sentences, the purpose of this experiment.

BIOS256 OL, Week 2 Lab Name: The purpose of this experiment is to understand how glucose is transported throughout our bodies. Observations: List 2 observations you have made in this simulation. Two observations from this simulation are: 1) Sodium levels control whether glucose is absorbed by the body. 2) When glucose is not absorbed in the body, it can cause diarrhea. Answer all the questions below: 1. How does sodium regulate the uptake of glucose into enterocytes? (3 points) Sodium regulates the uptake of glucose into the enterocytes by way of a sodium glucose transporter. It absorbs 2Na+ and one glucose molecule using secondary active transport. 2. In the simulation, why did the infant experience diarrhea due to glucose malabsorption? (3 points) Infants/Children with Congenital Glucose Galactose Malabsorption (CGGM) bodies cannot absorb glucose and galactose. Therefore the excessive amounts of liquids that cannot be absorbed results in watery diarrhea. 3. What causes lactose intolerance? What are the products of lactose breakdown? (2 points) Lactose intolerance is caused by very low levels of lactase in the body. Lactase is an enzyme that helps the body digest lactose. Lactase breaks down into 2 simple sugars, glucose and galactose. 4. Why is the lumen of the GI tract lined by mucosal membrane? (2 points) The lumen of the GI tract is lined with mucosal membrane to help protect the organs from harmful pathogens and particles and lubricates the lumen. 5. Reflection: Reflect on at least 2 key concepts you have learned from this simulation. How can you apply it to the physiological processes in the body?

BIOS256 OL, Week 2 Lab Name: Grading Rubric: Activity Deliverable Points Part 1 Complete simulation 15 Part 2 Complete lab report and answer questions  Purpose (1 point)  Observation (2 points)  Questions (10 points)  Reflection (2 points) 15 Total Complete all lab activities 30

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