Standard Curve Assignment
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websteruniv.edu *
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Course
141
Subject
Mathematics
Date
Apr 3, 2024
Type
docx
Pages
2
Uploaded by JusticeStar43869
1.
A. Using the class absorbance data collected for the concentrations of betacyanin:
a.
Calculate the mean and standard error for each betacyanin concentration analyzed. b.
Make a complete figure
using the mean values +/- standard error following the guidelines in
Appendix C of the lab manual. To facilitate using this figure as a standard curve, be sure to include the best fit straight line
of the data, including the equation of the line
.
c.
Paste your standard curve figure (graph and figure caption) below. (10)
0
2
4
6
8
10
12
14
16
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
f(x) = 0.06 x − 0.01
Betacyanin concentration (nM)
Average wavelength Absorbed (%)
Figure 1. Percentage of absorbance increases as the wave lengths increase. There was a liner increase throughout the whole graph the, but there was a slight deviation around the betacyanin concentration mark 12. However, the percent increase from concentration 2 to 12 is a 393% increase and from 12 to 14 it is a 34% increase. (Mean
SE, n=6)
d.
If the absorbance of betacyanin was 0.25 at λmax, what was the concentration of the solution? Use the equation of the line from your
standard curve (y=mx+b) to interpolate and answer this question. Show your work for full credit. (3)
Y=0.0566(x)-.0131
.25=0.0566(x)-.0131
+.0131. +.0131
.2631/.0566=.0566(x) /.0566
4.65=x
e.
If λmax for a 2
µ
M betacyanin solution was 535nm, what would λmax be for a 6
µ
M solution of betacyanin? (1)
The max for everything is 535nm. f.
Explain your answer to #3. (1)
This is because once it reaches 535 there is not a higher point in which more light can be absorbed that is the maximum.
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