CHEM 230L_TLC and column chromatography

Identify the detectors in gas chromatography that can be used for the detection of each type of analyte. More than one detector may be appropriate for a given analyte, and a given detector may be appropriate for more than one type of analyte. compounds ionized by UV radiation sulfur or nitrogen containing compounds halogenated compounds flame ionization Answer Bank mass spectrometer nitrogen and phosphorous containing compounds thermal conductivity electrolytic conductivity hydrocarbons electron capture thermionic photoionization

How else can chromatography be used?   None of these answers are correct.   To wash (or remove) unwanted solvents from a mixture.   To identify or exclude components in a mixture.   To generate IUPAC names for hydrocarbons.

19. What is thin layer chromatography (TLC) LEAST useful for from the list below? A) To determine if crystallization or distillation may be more effective for a separation B) To determine the number of components in a mixture C) To determine the appropriate solvent for column- chromatographic separation D) To monitor the progress of an organic synthesis reaction 14. The solubility of salicylic acid in water is 7.8g/100ml at 100°C and 0.25g/100ml at 25°. Estimate how much water is needed to recrystallize a 19g sample of salicylic acid? A) 150ml B) 250ml C) 300ml D) 400ml

For part B, it is not 4.98 x 10-41. I put it in and it was wrong. It is looking for P1.

Explain the following Chromatography (for drug analysis) Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC)

PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.

Question: Can thin-layer chromatography (TLC) be used to differentiate between two closely related compounds with similar structures?

1. How does chromatography separate the components of leave samples? 2. Can you use chromatography to purify a mixture? why? 3. What is Rf value and its significance in chromatography?

Please don't provide handwriting solution

Analysis of pure molecules either heterogenous mixture or analysis of pure molecules neither heterogenous mixture nor analysis of pure molecules heterogenous mixture

Absorbance 1.40 Beer's Law Plot 1.20 1.00 0.80 0.60 0.40 0.20 0.00 0 10 20 30 40 50 Concentration (M x 105) D The slope for the line is 80.23. Use the Beer's Law plot provided to determine the concentration for a solution with absorbance = 0.5786. M

Pre-Lab Questions 1. Define a protein, and describe one important function of proteins in the human body. 2. The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (ug) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 Use Excel, another program, or graph paper to produce a calibration curve based on the data. Use linear regression (or best fit line) to obtain the slope of the line. Determine the unknown protein content for a sample that shows an absorbance reading of b. С. 0.150.

Please choose an appropriate technique(s) to separate following compounds (it can be one, more than one, on none): Compound Melting Point Density Boiling Point Molar mass Phenol 40.5°C 1.07 g/cm 181.7°C 94.113 g/mol Toluene -95 °C 0.87 g/mL 111 °C 92.141 g/mol Simple Filtration Column Chromatography Distilation Crystallization/Re-Crystallization Separatory funnel None www. MacBook Air

The paper used in chromatography is made of molecules with___parts A polar  B nonpolar

Question:  Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC.  -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.

S (CQCL3) t σ 200 180 160 140 120 100 80 20 PPM 0 S (CDC13) 9 200 180 160 140 120 100 80 60 40 20 PPM 0 NH₂ T OH .OH །ཚིགས་.. OH

Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.

1. Take your data home and use EXCEL to prepare a Beer's Law plot of the data from your standards (Trials 1-5). You should plot Absorbance on the "y" axis and concentration on the "x" axis. Include a print out of your graph with your report. 2. Add a trend line to your graph in the same way that you added the trend line to the graph you prepared in your density experiment (EXP 1). Be sure to show the equation the line on your graph. 3. Utilize your graph to determine the concentration of your unknown copper sulfate solution. Show how you did this on your graph. Unknown Concentration from Graph. M 4. Use the equation of the line on your graph to determine the concentration of your unknown copper sulfate solution. Show your work below. Unknown concentration from linear equation M