TLC Procedure and Post-Lab

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School

Florida International University *

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3501

Subject

Chemistry

Date

Feb 20, 2024

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CHS 3501L TLC Procedure Objective: To understand Thin Layer Chromatography and how it can be used to separate components that comprise water soluble inks and markers. Materials: - Three water-soluble inks or markers - Paper/Coffee Filter - Hand sanitizer mixed with water (50%) Procedure: 1. Obtain three sources of water-soluble ink. Record detailed information in your notes about the color and appearance for each of the known samples. 2. Using a plain 8.5” X 11” paper, draw a line in pencil across the page. Mark the inks on that penciled line as in the below example. 3. Place the paper within a small cup with a small amount of the hand sanitizer/water mixture (see photo). Do not allow the mixture to touch the spots you drew. Stand the paper up against a wall or hang using a paper clip for 20-60 minutes, allowing the hand sanitizer/water mixture to move up the paper using capillary action. Be sure the liquid does not go off the paper. Coffee filter paper may require significantly less time. 4. Remove the paper and allow the spots to dry. 5. Trace the spots or bands lightly with a pencil. Trace the solvent front with a pencil. Attach a photo. 6. Measure all distances and calculate R f values for each color component. Distances can be measured to top, middle, or bottom of spot. Just be consistent for all results. Record your measurements and calculations using the following table as a template. Add rows if needed. 1 2 3

CHS 3501L TLC Laboratory and Post-Lab Sample Band Color Distance Covered by Band (cm) Distance Covered by Solvent Front (cm) R f Value Please answer the following questions. 1. Did the three samples look like each other? Explain. (6 pts) 2. Which ink/marker had the greatest distance traveled? Did this bind more strongly to the stationary phase or to the mobile phase? Justify your answers. (2 pts) 3. A ransom note was found at the scene of a kidnapping. Describe in detail how you would analyze this piece of evidence to gather more clues about the case and what information it might provide. (2 pts)

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