Spinach Extractions - Lab Report 3

3. Using the data at right: a. Calculate the absorbance of each sample. You will need to express the %T values as decimals before calculating the logarithms. (e.g., for T = 25%, A = -log 0.25 = 0.602) b. Use the graph paper on the back of this page (or a spreadsheet) to plot absorbance vs. concentration. Make a proper graph, with labeled axes, uniform scales, etc. Use a ruler to draw the best-fit line.. C. Use the graph to find the concentration of the un- known sample. (Draw dotted lines on the graph showing how you found your answer.) CONCENTRATION PERCENT (ppm) TRANSMISSION 0 100.0 100 74.1 200 54.9 300 40.7 400 30.1 500 22.3 unknown 31.6 ABSORBANCE

Project 2: Food Dye Spectroscopy   How can you use serial dilutions to determine the relationship between concentration and absorbance? Graphs may also help in this explanation.

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This question uses the same data as the previous question. The calibration curve is a graph of absorbance versus concentration. Absorbance is a dimensionless quantity. The slope of the calibration curve is m = 2.24 x 10^4 M^-1. Find the concentration detection limit.

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Please solve both sub-parts accurate and exact answers please it's very important!! I'll rate it up if accurate

Please complete Table 2 and show the calculations for protein concentration and the concentration of the undiluted sample.  a). Using Excel software, enter data from Table 1 to plot a standard curve (corrected average absorbance on Y-axis and protein concentrations on X-axis). The standard curve must contain correct labels on both axes. b). From the standard curve, obtain a best fit line and equation. c). Use the best fit equation and data from Table 2 to find the concentrations of proteins in sample A2-D2 and E1.  [onto your plotted standard curve, indicate where A595 of A2-D2 and E1 are located]  d). Calculate the concentrations of proteins in undiluted samples A to E. Thank you very much.

* Question Completion Status: QUESTION 1 What is the sample absorbance of a solution having a percent transmittance of 84.2%? O a. 1.93 O b.0.801 O c. 0.0747 O d.0.158 Click Save and Submit to save and submit. Click Save All Answers to save all answers. search 21

3. If you wished to study the molecule below by measuring absorbance, what wavelength should you set your spectrophotometer to? 1.6 Absorbance 1.4 1.2 1.0 0.8 0.6 0.4- 0.2- ME 0.0 300 400 600 Wavelength (nm) 500 Solutions and Mol....pdf 2 700 20221027_141317.jpg 800

Calculate the concentration of the unknown MnO4 sample, given the following standard addition experiment. Step 1: A 2.00 mL aliquot of an unknown MnO4 sample was diluted to 100.0 mL and the absorbance measured to be 0.275. Step 2: A 2.00 mL aliquot of an unknown MnO4 sample was placed in a 100.0 mL flask. A 2.00 mL aliquot of a 100.00 ppm MnO4 standard was added to the flask and the absorbance measured to be 0.6. Report your answer to one decimal place.

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Project 2: Food Dye Spectroscopy   Which scale is most useful for reporting data from the spectrometer? Why?

Two samples were analyzed using a colormeter on a cellphone: one containing only distilled water, and another containing an unknown solution. The distilled water sample had a Red value of 206, while the unknown solution had a Red value of 118. Calculate the absorbance of the unknown solution. -log (4) A = -log a) -0.242 b) 0.573 c) 0.242 d) 0.000 CASAT

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Timer Course Contents » ... » Data, Statistics, and Graphing » Graphing Absorbance (Optical Density) versus Molar Concentration The following table contains some data showing the effect of molar concentration of a solution on the absorbance (optical density) reading from a spectrophotometer. No ↑ Does the solution in your graph follow Beer's Law? Concentration (M) Submit Answer Incorrect. Tries 37/99 Previous Tries 0.13 0.28 0.43 0.58 0.73 0.88 1.03 1.18 1.33 1.48 What is the concentration of a solution that has an Optical Density of 0.21? (Give units in your answer). 0.21M You are correct. Your receipt no. is 155-7520 ? What is the concentration of a solution that has an Optical Density of 0.91? (Give units in your answer). 0.81 M Previous Tries Optical Density To assist in graphing, right click on this excel spreadsheet file, save it to your computer, and open it in Excel. Enter the above data in the appropriate spaces in the spreadsheet. Select the graph that is created, and print…

Tech X Mail - Jackson Illingworth - Outl X Blackboard Learn oblearn.atu.edu/ultra/courses/_32419 1/cl/outline QUESTION 2 What is the sample absorbance of a solution having a transmittance of 65.2%? O a. 0.186 O b. 1.81 O c. 0.458 O d. 0.348 QUESTION 3

Determine the standard concentration of A through E and then the absorbance. I just need clarification on if I add together the two ml values given.

Question 19 of 27 > Graphs are a common data analysis tool used in all branches of science. The given graph represents how light absorbance at 423 nm varies with concentration of an organic dye. Identify the parts of the graph. Answer Bank y = 0.210x – 0.032 line equation Title Abs423 Dye Conc. (nM) best-fit line 0 i 2 3 4 5 6 7 8 9 10

To determine the amount of copper and the identity of the copper compound in the unknown sample, a 100-mL solution containing 120.8 mg of the sample was initially prepared. Then, 5.00 mL of this solution was pipetted and diluted with deionized water in another 100-mL volumetric flask. The absorbance of this diluted solution was determined three times at the analytical wavelength. The data is in the table below.    Unknown sample Trial 1 Trial 2 Trial 3 Absorbance (AU) 0.378 0.379 0.377 What is the corrected absorbance?