Unit 2, HW4_ Hematocrit

.pdf

School

National University College *

*We aren’t endorsed by this school

Course

ANALYTICAL

Subject

Chemistry

Date

Jun 6, 2024

Type

pdf

Pages

5

Uploaded by BrigadierApe2137

1/11/23, 11:02 PM Unit 2, HW4: Hematocrit https://session.masteringaandp.com/myct/assignmentPrintView?assignmentID=9492065 1/5 Unit 2, HW4: Hematocrit Due: 11:59pm on Wednesday, January 11, 2023 You will receive no credit for items you complete after the assignment is due. Grading Policy Pre-lab Video Coaching Activity: Determining the Hematocrit Watch the following Pre-lab Video: Determining the Hematocrit and complete the activity below. Part A - Composition of Whole Blood Drag the labels to identify the components of whole blood after centrifugation. Hint 1. Erythrocytes
1/11/23, 11:02 PM Unit 2, HW4: Hematocrit https://session.masteringaandp.com/myct/assignmentPrintView?assignmentID=9492065 2/5 Erythrocytes, or red blood cells (RBCs), have iron-containing hemoglobin that transports oxygen and carbon dioxide. Do you think the iron in the RBCs makes them lighter or heavier than the other components of whole blood? Hint 2. What is the hematocrit? The hematocrit is the percentage of RBCs out of the total blood volume. Hint 3. Buffy coat composition The blood contains three types of formed elements: erythrocytes, leukocytes, and platelets. The buffy coat contains two out of the three formed elements. ANSWER: Correct Plasma makes up 55% of the whole blood, and the formed elements (leukocytes, erythrocytes, and platelets) make up the remaining 45%. Part B - Determining the Hematocrit Recall from the video the steps for determining the hematocrit. Place the steps in the correct order from left to right. Hint 1. What is the last step in determining the hematocrit? Recall that the hematocrit is the percentage of RBCs out of the total blood volume. In order to separate the components of whole blood and determine the hematocrit, you must centrifuge the blood sample. Hint 2. What does a centrifuge do? A centrifuge separates particles according to their density. The dense (heavier) particles move toward the wall and the lighter ones remain close to the center. The tubes in the microhematocrit centrifuge are angled outward slightly, so heavier particles will sink to the bottom of the tube. Reset Help Plasma Buffy coat Leukocytes Platelets Erythrocytes Hematocrit Top layer Middle layer Bottom layer
1/11/23, 11:02 PM Unit 2, HW4: Hematocrit https://session.masteringaandp.com/myct/assignmentPrintView?assignmentID=9492065 3/5 ANSWER: Correct A hematocrit can be used to diagnose and monitor rheumatoid arthritis, anemia, and several cancers. Part C - Abnormal Hematocrits to Identify Health Conditions Compare the normal hematocrit to the patient’s hematocrit. What is the patient’s likely condition based upon the hematocrit shown? Reset Help Place heparinized capillary tube into the blood. Fill the tube three-quarters full with blood. Seal the end with capillary tube sealer or clay. Place the tube in a microhematocrit centrifuge. Centrifuge the blood for 4 to 5 minutes. Determine the percent of RBCs.
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help